Immunopathology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Washington, DC, USA.
Histol Histopathol. 2011 Sep;26(9):1145-51. doi: 10.14670/HH-26.1145.
Sympathetic ophthalmia is a bilateral uveitis that develops after penetrating injury to one eye. This study aimed to identify the inflammatory cellular sub-phenotypes and expression of pertinent inflammatory cytokines/chemokines in sympathetic ophthalmia (SO). Dalen-Fuchs nodules (DFN), granulomas, and non-granulomatous foci of inflammation were micro-dissected from 15 cases. RNA was extracted, and quantitative PCR was performed to measure IL-17, IL-18, IL-23, IFN-γ, CCL19, CXCL11, CCL17, and CCL22 transcripts. Immunohistochemical methods were used to characterize CD3, CD4, CD8, CD20, CD68, and CD163 expression. Non-granulomatous lymphocytes were predominantly CD3-positive and expressed more IFN-γ than cells within granulomas, consistent with Th1 cells. In contrast, granulomas and DFN contained mainly CD68+, CD163+/- and expressed more IL-17, IL-18, IL-23, CCL19, and CXCL11 than non-granulomatous cells. Our data indicate for the first time that M1 macrophages are the predominant inflammatory cells within granulomas and DFN of SO. We further observed high levels of IL-17 within granulomas and the presence of Th1 and M1 cells.
交感性眼炎是一种双眼葡萄膜炎，发生于一眼穿透性损伤后。本研究旨在确定交感性眼炎（SO）中的炎症细胞亚表型和相关炎症细胞因子/趋化因子的表达。从 15 例中分离出 Dalen-Fuchs 结节（DFN）、肉芽肿和非肉芽肿性炎症灶。提取 RNA，进行定量 PCR 测量 IL-17、IL-18、IL-23、IFN-γ、CCL19、CXCL11、CCL17 和 CCL22 转录本。采用免疫组织化学方法检测 CD3、CD4、CD8、CD20、CD68 和 CD163 的表达。非肉芽肿性淋巴细胞主要为 CD3 阳性，表达的 IFN-γ多于肉芽肿内细胞，与 Th1 细胞一致。相比之下，肉芽肿和 DFN 主要包含 CD68+、CD163+/-，并表达更多的 IL-17、IL-18、IL-23、CCL19 和 CXCL11，而不是非肉芽肿性细胞。我们的数据首次表明，M1 巨噬细胞是 SO 中肉芽肿和 DFN 中的主要炎症细胞。我们进一步观察到在肉芽肿内存在高水平的 IL-17 以及 Th1 和 M1 细胞的存在。
