Liu Qian, Zhang Xiao-Lin, Tao Rong-Ya, Niu Ya-Jing, Chen Xiao-Guang, Tian Jin-Ying, Ye Fei
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
J Asian Nat Prod Res. 2011 Aug;13(8):714-23. doi: 10.1080/10286020.2011.586341.
Rhein (RH), a compound purified from Radix et Rhizoma Rhei, has been used to alleviate liver and kidney damage. It is found that RH inhibited the differentiation of 3T3-L1 preadipocytes induced by differentiation medium in a time- and dose-dependent manner. It was revealed that RH downregulated the expression of adipogenesis-specific transcription factors PPARγ and C/EBPα, as well as their upstream regulator, C/EBPβ. Furthermore, the PPARγ target genes that are involved in adipocyte differentiation, such as CD36, aP2, acyl CoA oxidase, uncoupled protein 2, acetyl-CoA carboxylase, and fatty acid synthase, were reduced after to RH. In addition, high-fat diet-induced weight gain and adiposity were reversed by RH in C57BL/6 mice. Consistent with the cells' results, RH downregulated the mRNA levels of PPARγ and C/EBPα, and their downstream target genes in C57BL/6 mice. Taken together, adipocyte differentiation and adipogenesis were inhibited by RH in cultured cells and in rodent models of obesity. The evidence implied that RH was a potential candidate for preventing metabolic disorders.
大黄酸（RH）是从大黄根茎中提纯的一种化合物，已被用于减轻肝肾损伤。研究发现，RH以时间和剂量依赖性方式抑制分化培养基诱导的3T3-L1前脂肪细胞分化。结果表明，RH下调脂肪生成特异性转录因子PPARγ和C/EBPα及其上游调节因子C/EBPβ的表达。此外，在给予RH后，参与脂肪细胞分化的PPARγ靶基因，如CD36、aP2、酰基辅酶A氧化酶、解偶联蛋白2、乙酰辅酶A羧化酶和脂肪酸合酶均减少。此外，RH可逆转高脂饮食诱导的C57BL/6小鼠体重增加和肥胖。与细胞实验结果一致，RH下调了C57BL/6小鼠中PPARγ和C/EBPα及其下游靶基因的mRNA水平。综上所述，在培养细胞和肥胖啮齿动物模型中，RH均可抑制脂肪细胞分化和脂肪生成。这一证据表明，RH是预防代谢紊乱的潜在候选药物。
