MPL, Meikai University School of Dentistry, Sakado, Saitama 350-0283, Japan.
In Vivo. 2011 Sep-Oct;25(5):751-5.
We have previously reported several lignin-like activities of the alkaline extract of the leaves of Sasa senanensis Rehder (SE), such as anti-HIV and radical scavenging activity. As an extension, possible SE protection of cells from ultraviolet (UV)-induced injury (referred to "anti-UV activity") was investigated.
HSC-2, human oral squamous cell carcinoma cells, were exposed to UV irradiation in phosphate-buffered saline containing SE or its fractions, and then incubated for 48 hours in fresh regular culture medium to determine the viable cell number by the MTT method. SE was separated by gel filtration chromatography into the following four fractions: polysaccharide, large and small lignin-carbohydrate complexes and lower molecular weight polyphenol fractions (Fr. I).
The anti-UV activity of SE was slightly less than that of sodium ascorbate, but higher than that of gallic acid, (-)-epigallocatechin gallate, chlorophyll a and chlorophyllin. The combination of SE and sodium ascorbate gave synergistic anti-UV activity. On gel filtration fractionation, the majority of the anti-UV activity was recovered from Fr. I, which was eluted as a single peak on high performance liquid chromatography (HPLC).
The anti-UV activity of SE further suggests its potential as an alternative medicine.
我们之前曾报道过 SasasenanensisRehder（SE）叶的碱性提取物具有几种木质素样活性，如抗 HIV 和自由基清除活性。作为扩展，研究了 SE 对细胞免受紫外线（UV）诱导损伤的可能保护作用（称为“抗 UV 活性”）。
将 HSC-2，人口腔鳞状细胞癌细胞，在含有 SE 或其级分的磷酸盐缓冲盐水（PBS）中暴露于 UV 照射下，然后在新鲜常规培养基中孵育 48 小时，通过 MTT 法测定活细胞数。SE 通过凝胶过滤色谱法分离成以下四个级分：多糖、大、小木质素-碳水化合物复合物和低分子量多酚级分（Fr.I）。
SE 的抗 UV 活性略低于抗坏血酸钠，但高于没食子酸、(-)-表没食子儿茶素没食子酸酯、叶绿素 a 和叶绿素啉。SE 和抗坏血酸钠的组合具有协同抗 UV 活性。在凝胶过滤分级中，大多数抗 UV 活性从 Fr.I 中回收，该级分在高效液相色谱（HPLC）中作为单个峰洗脱。
SE 的抗 UV 活性进一步表明其作为替代药物的潜力。
