Division of Pharmacology, Meikai University School of Dentistry, Sakado, Saitama 350-0283, Japan.
In Vivo. 2012 May-Jun;26(3):411-8.
We have previously reported that alkaline extract of Sasa senanensis leaves (SE) showed potent anti-HIV, anti-UV and radical scavenging activity. In the present study, we investigated the biological activities of SE-10, a granulated powder of SE supplemented with lactose, lactitol, trehalose and tea extract.
Cell viability of mock-infected, HIV-infected, and UV-irradiated cells was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Scavenging activity of superoxide anion and hydroxyl radicals was determined by electron-spin resonance spectroscopy. Cytochrome P-450 (CYP)3A4 activity was measured by β-hydroxylation of testosterone in human recombinant CYP3A4.
SE-10 had slightly higher anti-HIV and anti-UV activities, but slightly lower radical-scavenging and CYP3A4-inhibitory activities, as compared with SE.
The present study demonstrates that the biological activities of SE were well preserved during the manufacturing process of SE-10.
我们之前曾报道过，紫萁科植物（Sasa senanensis）叶片的碱性提取物（SE）具有很强的抗 HIV、抗 UV 和自由基清除活性。在本研究中，我们研究了 SE-10 的生物学活性，SE-10 是一种 SE 颗粒粉末，添加了乳糖、乳糖醇、海藻糖和茶提取物。
通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法测定模拟感染、HIV 感染和 UV 照射细胞的细胞活力。通过电子自旋共振光谱法测定超氧阴离子和羟基自由基的清除活性。用人重组 CYP3A4 的β-羟化作用测量细胞色素 P-450（CYP）3A4 活性。
SE-10 具有稍高的抗 HIV 和抗 UV 活性，但自由基清除和 CYP3A4 抑制活性稍低。
本研究表明，SE 的生物学活性在 SE-10 的制造过程中得到了很好的保留。
