MPL, Meikai University School of Dentistry, Sakado, Saitama, Japan.
In Vivo. 2011 Sep-Oct;25(5):757-62.
In contrast to the several reports of alkaline extracts (Sasa-health, SE), no study of flavonoids from the leaves of S. senanensis has been reported. Four flavonoids were isolated from this plant species and their biological activities were investigated.
Luteolin 6-C-β-D-glucoside [1], luteolin 7-O-β-D-glucoside [2], luteolin 6-C-α-L-arabinoside [3] and tricin [4] were extracted from the leaf of S. senanensis with methanol, partitioned with ethyl acetate, separated by Sephadex LH-20 and purified by high-performance liquid chromatography (HPLC). The structure was determined by ultraviolet (UV) spectra, high-resolution mass spectra (HR-MS) and nuclear magnetic resonance (NMR).
The luteolin glycosides, 1-3 showed no cytotoxicity against the human normal oral cells and oral squamous cell carcinoma cell lines used up to 0.8 mg/ml, whereas 4 was highly cytotoxic. The luteolin glycosides 1-3 protected the cells from UV induced cytotoxicity, more efficiently than 4. The anti-HIV activity of 4 (Selectivity index, SI=27) was much higher than that of the luteolin glycosides (SI=2-7), but lower than that of SE (SI=40). The scavenging activity of 1-3 against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion radicals was comparable with that of quercetin and, much higher than that of 4.
The luteolin glycosides from S.senanensis show several new biological properties distinct from tricin and the anti-UV activity of the luteolin glycosides may be derived from their radical scavenging activity.
与几种关于碱性提取物（Sasa-health，SE）的报道形成对比的是，目前尚未有关于华西银腊梅叶片中类黄酮的研究报道。本研究从该植物物种中分离得到 4 种类黄酮，并对其生物活性进行了研究。
采用甲醇从华西银腊梅叶片中提取木樨草素 6-C-β-D-葡萄糖苷[1]、木樨草素 7-O-β-D-葡萄糖苷[2]、木樨草素 6-C-α-L-阿拉伯糖苷[3]和三萜[4]，用乙酸乙酯萃取，经 Sephadex LH-20 柱层析分离，并用高效液相色谱（HPLC）纯化。通过紫外（UV）光谱、高分辨质谱（HR-MS）和核磁共振（NMR）确定其结构。
木樨草苷 1-3 对人正常口腔细胞和口腔鳞状细胞癌细胞系的细胞毒性作用在 0.8mg/ml 以下，而 4 则具有很高的细胞毒性。与 4 相比，木樨草苷 1-3 更有效地保护细胞免受 UV 诱导的细胞毒性。4 的抗 HIV 活性（选择性指数，SI=27）明显高于木樨草苷（SI=2-7），但低于 SE（SI=40）。1-3 对 1,1-二苯基-2-苦基肼（DPPH）和超氧阴离子自由基的清除活性与槲皮素相当，明显高于 4。
华西银腊梅中的木樨草苷具有几种与三萜不同的新生物学特性，木樨草苷的抗 UV 活性可能源于其自由基清除活性。
