Fukuchi Kunihiko, Sakagami Hiroshi, Sugita Yoshiaki, Takao Koichi, Asai Daisuke, Terakubo Shigemi, Takemura Hiromu, Ohno Hirokazu, Horiuchi Misaki, Suguro Madoka, Fujisawa Tomohiro, Toeda Kazuki, Oizumi Hiroshi, Yasui Toshikazu, Oizumi Takaaki
Graduate School of Health Sciences, Showa University, Hatanodai 1-5-8, Shinagawa, Tokyo 142-8555, Japan.
Research Institute of Odontology (M-RIO), Meikai University, Keyakidai 1-1, Sakado, Saitama 350-0283, Japan.
Medicines (Basel). 2020 Oct 6;7(10):64. doi: 10.3390/medicines7100064.
Herpes simplex virus (HSV) is usually dormant and becomes apparent when body conditions decline. We investigated the anti-HSV activity of various natural and synthetic compounds for future clinical application. Mock- and HSV-infected Vero cells were treated for three days with various concentrations of samples. For short exposure, 100-fold concentrated virus were preincubated for 3 min with samples, diluted to normal multiplicity of infection (MOI), before the addition to the cells. Anti-HSV activity was evaluated by the chemotherapy index. Alkaline extracts of the leaves of sp. (SE) and pine cone (PCE) showed higher anti-HSV activity than 20 Japanese traditional herb medicines (Kampo formulas), four popular polyphenols, and 119 chromone-related compounds. Exposure of HSV to SE or PCE for 3 min almost completely eliminated the infectivity of HSV, whereas much longer exposure time was required for Kakkonto, the most active Kampo formulae. Anti-HSV activity of PCE and Kakkonto could be detected only when they were dissolved by alkaline solution (pH 8.0), but not by neutral buffer (pH 7.4). Anti-HSV activity of SE and povidone iodine was stable if they were diluted with neutral buffer. The present study suggests the applicability of SE and PCE for treatment of oral HSV and possibly other viruses.
单纯疱疹病毒（HSV）通常处于潜伏状态，在身体状况下降时会显现出来。我们研究了各种天然和合成化合物的抗HSV活性，以备将来临床应用。用不同浓度的样品对未感染和感染HSV的Vero细胞进行了三天的处理。对于短时间暴露，将100倍浓缩的病毒与样品预孵育3分钟，稀释至正常感染复数（MOI），然后加入细胞。通过化疗指数评估抗HSV活性。sp.叶的碱性提取物（SE）和松果体提取物（PCE）显示出比20种日本传统草药（汉方配方）、四种常见多酚和119种色酮相关化合物更高的抗HSV活性。将HSV暴露于SE或PCE 3分钟几乎完全消除了HSV的感染性，而对于活性最高的汉方配方葛根汤，则需要更长的暴露时间。只有当PCE和葛根汤用碱性溶液（pH 8.0）溶解时才能检测到抗HSV活性，而用中性缓冲液（pH 7.4）则检测不到。如果用中性缓冲液稀释，SE和聚维酮碘的抗HSV活性是稳定的。本研究表明SE和PCE可用于治疗口腔HSV以及可能的其他病毒。
