Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Poult Sci. 2011 Aug;90(8):1728-36. doi: 10.3382/ps.2011-01354.
We studied the effects of dietary oxidized oils on serum lipid metabolic indices, estradiol level, and the gene expression of apolipoprotein B-100 and apolipoprotein VLDL-II in laying hens. Hy-Line Grey hens (280 ± 10 d old; average egg production, 90.0 ± 2.5%) were allotted to 1 of 4 dietary treatments, which were supplemented with 0 (control group), 1% (low oxidized group), 2% (moderately oxidized group), or 4% (highly oxidized group) thermally oxidized soybean oil. Each treatment contained 6 replicates, with 12 birds each. The feeding trial lasted for 30 d. Laying performance data were recorded weekly. Other indices were measured on d 0, 2, 6, 14, and 30 of the feeding trial. Hens in the moderately and highly oxidized groups had significantly lowered feed conversion ratios (P < 0.05). Those in the highly oxidized group also had decreased concentrations of serum very low density lipoprotein cholesterol on d 30 (P < 0.05) compared with the very low density lipoprotein cholesterol of hens in the moderately oxidized group. Hens in the moderately oxidized group had significantly increased expression of apolipoprotein B-100 (P < 0.05) from d 6 to 30. Consequently, hepatic triglyceride increased in this group on d 30 (P < 0.05). Serum triglyceride decreased in the moderately oxidized group on d 30 (P < 0.05), which may have been caused by the activation of peroxisome proliferator-activating receptor α. Serum estradiol levels were not significantly affected by oxidized oils at any time of measurement, but were significantly different between d 0 and 30 within the moderately oxidized group. This fact indicated that the effect of oxidized oils on apolipoprotein B-100 might partially be a cumulative result of the increasing secretion of estradiol. The results suggested that oxidized oil may affect the performance of laying hens through the regulation of apolipoproteins and estradiol.
我们研究了日粮氧化油对产蛋鸡血清脂质代谢指标、雌二醇水平以及载脂蛋白 B-100 和载脂蛋白 VLDL-II 基因表达的影响。选用 280±10 日龄的海兰灰蛋鸡（产蛋率 90.0±2.5%），随机分为 4 个日粮处理组，分别添加 0（对照组）、1%（低氧化组）、2%（中氧化组）或 4%（高氧化组）热氧化大豆油。每个处理组设 6 个重复，每个重复 12 只鸡。试验期 30 d。每周记录产蛋性能数据。其他指标在试验第 0、2、6、14 和 30 天进行测定。中氧化组和高氧化组的母鸡的饲料转化率显著降低（P＜0.05）。与中氧化组相比，高氧化组的母鸡在第 30 天的血清极低密度脂蛋白胆固醇浓度也显著降低（P＜0.05）。从第 6 天到第 30 天，中氧化组母鸡的载脂蛋白 B-100 表达显著增加（P＜0.05）。因此，第 30 天该组母鸡的肝甘油三酯增加（P＜0.05）。第 30 天，中氧化组母鸡的血清甘油三酯降低（P＜0.05），这可能是过氧化物酶体增殖物激活受体α被激活的结果。氧化油在任何测量时间都不会显著影响血清雌二醇水平，但在中氧化组中，第 0 天和第 30 天之间的水平有显著差异。这一事实表明，氧化油对载脂蛋白 B-100 的影响可能部分是雌二醇分泌增加的累积结果。结果表明，氧化油可能通过调节载脂蛋白和雌二醇来影响蛋鸡的生产性能。
