Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei.
Int J Nanomedicine. 2011;6:1229-35. doi: 10.2147/IJN.S21019. Epub 2011 Jun 20.
Superparamagnetic iron oxide nanoparticles have been used in clinical applications as a diagnostic contrasting agent. Previous studies showed that iron oxide nanoparticles deposited in the liver and spleen after systemic administration. The present study investigated the effect of iron oxide nanoparticles on antigen-specific immune responses in mice sensitized with the T cell-dependent antigen ovalbumin (OVA).
BALB/c mice were intravenously administered with a single dose of iron oxide nanoparticles (10-60 mg Fe/kg) 1 hour prior to OVA sensitization, and the serum antibody production and splenocyte reactivity were examined 7 days later.
The serum levels of OVA-specific IgG(1) and IgG(2a) were significantly attenuated by treatment with iron oxide nanoparticles. The production of interferon-γ and interleukin-4 by splenocytes re-stimulated with OVA in culture was robustly suppressed in mice administered with iron oxide nanoparticles. The viability of OVA-stimulated splenocytes was also attenuated. In contrast, treatment with iron oxide nanoparticles did not affect the viability of splenocytes stimulated with concanavalin A, a T-cell mitogen.
Collectively, these data indicate that systemic exposure to a single dose of iron oxide nanoparticles compromises subsequent antigen-specific immune reactions, including the serum production of antigen-specific antibodies, and the functionality of T cells.
超顺磁性氧化铁纳米颗粒已被用作临床诊断造影剂。先前的研究表明，这些纳米颗粒在全身给药后会沉积在肝脏和脾脏中。本研究旨在探讨超顺磁性氧化铁纳米颗粒对卵清蛋白（OVA）致敏的小鼠中抗原特异性免疫反应的影响。
BALB/c 小鼠在 OVA 致敏前 1 小时静脉注射单次剂量的氧化铁纳米颗粒（10-60mg Fe/kg），7 天后检测血清抗体产生和脾细胞反应性。
氧化铁纳米颗粒处理显著降低了 OVA 特异性 IgG(1)和 IgG(2a)的血清水平。用 OVA 再刺激培养的脾细胞时，干扰素-γ和白细胞介素-4的产生被强烈抑制，经氧化铁纳米颗粒处理的小鼠脾细胞对 OVA 的刺激也减弱。相反，氧化铁纳米颗粒处理并不影响刀豆蛋白 A（一种 T 细胞有丝分裂原）刺激的脾细胞的活力。
综上所述，这些数据表明单次全身暴露于氧化铁纳米颗粒会损害随后的抗原特异性免疫反应，包括抗原特异性抗体的血清产生和 T 细胞的功能。
