Yurimoto Hiroya, Oku Masahide, Sakai Yasuyoshi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan.
Int J Microbiol. 2011;2011:101298. doi: 10.1155/2011/101298. Epub 2011 Jul 7.
Eukaryotic methylotrophs, which are able to obtain all the carbon and energy needed for growth from methanol, are restricted to a limited number of yeast species. When these yeasts are grown on methanol as the sole carbon and energy source, the enzymes involved in methanol metabolism are strongly induced, and the membrane-bound organelles, peroxisomes, which contain key enzymes of methanol metabolism, proliferate massively. These features have made methylotrophic yeasts attractive hosts for the production of heterologous proteins and useful model organisms for the study of peroxisome biogenesis and degradation. In this paper, we describe recent insights into the molecular basis of yeast methylotrophy.
真核甲基营养菌能够从甲醇中获取生长所需的所有碳和能量，这类菌仅限于少数几种酵母。当这些酵母在甲醇作为唯一碳源和能源的条件下生长时，参与甲醇代谢的酶会被强烈诱导，而含有甲醇代谢关键酶的膜结合细胞器过氧化物酶体则会大量增殖。这些特性使得甲基营养酵母成为生产异源蛋白的理想宿主，以及研究过氧化物酶体生物发生和降解的有用模式生物。在本文中，我们描述了对酵母甲基营养分子基础的最新见解。
