• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

[物种名称]的交替型（II型）线粒体NADH脱氢酶的生理相关性、定位及底物特异性

Physiological relevance, localization and substrate specificity of the alternative (type II) mitochondrial NADH dehydrogenases of .

作者信息

Juergens Hannes, Mielgo-Gómez Álvaro, Godoy-Hernández Albert, Ter Horst Jolanda, Nijenhuis Janine M, McMillan Duncan G G, Mans Robert

机构信息

Department of Biotechnology, Delft University of Technology, Delft, Netherlands.

School of Biological Sciences, University of Reading, Reading, United Kingdom.

出版信息

Front Microbiol. 2024 Dec 12;15:1473869. doi: 10.3389/fmicb.2024.1473869. eCollection 2024.

DOI:10.3389/fmicb.2024.1473869
PMID:39726963
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11670749/
Abstract

Mitochondria from harbor a branched electron-transport chain containing a proton-pumping Complex I NADH dehydrogenase and three Type II NADH dehydrogenases (NDH-2). To investigate the physiological role, localization and substrate specificity of these enzymes, the growth of various NADH dehydrogenase knockout mutants was quantitatively characterized in shake-flask and chemostat cultures, followed by oxygen-uptake experiments with isolated mitochondria. NAD(P)H:quinone oxidoreduction of the three NDH-2 were individually assessed. Our findings reveal that the respiratory chain contains an internal NADH-accepting NDH-2 (Ndh2-1/OpNdi1), at least one external NAD(P)H-accepting enzyme, and likely additional mechanisms for respiration-linked oxidation of cytosolic NADH. Metabolic regulation appears to prevent competition between OpNdi1 and Complex I for mitochondrial NADH. With the exception of OpNdi1, the respiratory chain of exhibits metabolic redundancy and tolerates deletion of multiple NADH-dehydrogenase genes without compromising fully respiratory metabolism.

摘要

来自[具体生物名称未给出]的线粒体含有一条分支的电子传递链，其中包括一个质子泵复合物I NADH脱氢酶和三种II型NADH脱氢酶（NDH-2）。为了研究这些酶的生理作用、定位和底物特异性，在摇瓶和恒化器培养中对各种NADH脱氢酶敲除突变体的生长进行了定量表征，随后对分离的线粒体进行了摄氧实验。分别评估了三种NDH-2的NAD(P)H:醌氧化还原作用。我们的研究结果表明，呼吸链包含一种内部NADH接受型NDH-2（Ndh2-1/OpNdi1）、至少一种外部NAD(P)H接受型酶，以及可能存在的用于呼吸相关的胞质NADH氧化的其他机制。代谢调节似乎可防止OpNdi1与复合物I之间对线粒体NADH的竞争。除OpNdi1外，[具体生物名称未给出]的呼吸链表现出代谢冗余，并且在不影响完全呼吸代谢的情况下能够耐受多个NADH脱氢酶基因的缺失。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc80/11670749/893b1dba6f9f/fmicb-15-1473869-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc80/11670749/893b1dba6f9f/fmicb-15-1473869-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc80/11670749/893b1dba6f9f/fmicb-15-1473869-g001.jpg

相似文献

1
Physiological relevance, localization and substrate specificity of the alternative (type II) mitochondrial NADH dehydrogenases of .[物种名称]的交替型（II型）线粒体NADH脱氢酶的生理相关性、定位及底物特异性
Front Microbiol. 2024 Dec 12;15:1473869. doi: 10.3389/fmicb.2024.1473869. eCollection 2024.
2
Contribution of Complex I NADH Dehydrogenase to Respiratory Energy Coupling in Glucose-Grown Cultures of .对葡萄糖生长的 培养物中呼吸能量偶联的复合物 I NADH 脱氢酶的贡献。
Appl Environ Microbiol. 2020 Jul 20;86(15). doi: 10.1128/AEM.00678-20.
3
Respiratory reoxidation of NADH is a key contributor to high oxygen requirements of oxygen-limited cultures of Ogataea parapolymorpha.NADH 的呼吸再氧化是限制供氧条件下聚多曲霉高需氧量的关键因素。
FEMS Yeast Res. 2022 Feb 22;22(1). doi: 10.1093/femsyr/foac007.
4
Type 2 NADH Dehydrogenase Is the Only Point of Entry for Electrons into the Streptococcus agalactiae Respiratory Chain and Is a Potential Drug Target.2 型烟酰胺腺嘌呤二核苷酸脱氢酶是电子进入酿脓链球菌呼吸链的唯一入口，也是一个潜在的药物靶点。
mBio. 2018 Jul 3;9(4):e01034-18. doi: 10.1128/mBio.01034-18.
5
The Saccharomyces cerevisiae NDE1 and NDE2 genes encode separate mitochondrial NADH dehydrogenases catalyzing the oxidation of cytosolic NADH.酿酒酵母的NDE1和NDE2基因编码独立的线粒体NADH脱氢酶，催化胞质NADH的氧化。
J Biol Chem. 1998 Sep 18;273(38):24529-34. doi: 10.1074/jbc.273.38.24529.
6
Expression of alternative NADH dehydrogenases (NDH-2) in the phytopathogenic fungus .植物病原真菌中交替型NADH脱氢酶（NDH-2）的表达
FEBS Open Bio. 2018 Jul 5;8(8):1267-1279. doi: 10.1002/2211-5463.12475. eCollection 2018 Aug.
7
In vivo analysis of the mechanisms for oxidation of cytosolic NADH by Saccharomyces cerevisiae mitochondria.酿酒酵母线粒体对胞质NADH氧化机制的体内分析。
J Bacteriol. 2000 May;182(10):2823-30. doi: 10.1128/JB.182.10.2823-2830.2000.
8
Genetic and Biochemical Analysis of Anaerobic Respiration in Bacteroides fragilis and Its Importance .脆弱拟杆菌厌氧呼吸的遗传和生化分析及其重要性。
mBio. 2020 Feb 4;11(1):e03238-19. doi: 10.1128/mBio.03238-19.
9
Enzymatic production of D-3-phenyllactic acid by Pediococcus pentosaceus D-lactate dehydrogenase with NADH regeneration by Ogataea parapolymorpha formate dehydrogenase.通过戊糖片球菌 D-乳酸脱氢酶和奥格门丁假丝酵母甲酸盐脱氢酶再生 NADH 酶促生产 D-3-苯乳酸。
Biotechnol Lett. 2014 Mar;36(3):627-31. doi: 10.1007/s10529-013-1404-2. Epub 2013 Nov 19.
10
The branched mitochondrial respiratory chain from Debaryomyces hansenii: components and supramolecular organization.汉逊德巴利酵母的分支线粒体呼吸链：组成成分与超分子组织
Biochim Biophys Acta. 2014 Jan;1837(1):73-84. doi: 10.1016/j.bbabio.2013.07.011. Epub 2013 Aug 7.

本文引用的文献

1
The Profound Influence of Lipid Composition on the Catalysis of the Drug Target NADH Type II Oxidoreductase.脂质组成对药物靶点NADH II型氧化还原酶催化作用的深远影响
Membranes (Basel). 2021 May 17;11(5):363. doi: 10.3390/membranes11050363.
2
Peripheral Membrane Proteins: Promising Therapeutic Targets across Domains of Life.外周膜蛋白：跨生命领域的有前景的治疗靶点。
Membranes (Basel). 2021 May 8;11(5):346. doi: 10.3390/membranes11050346.
3
Contribution of Complex I NADH Dehydrogenase to Respiratory Energy Coupling in Glucose-Grown Cultures of .
对葡萄糖生长的 培养物中呼吸能量偶联的复合物 I NADH 脱氢酶的贡献。
Appl Environ Microbiol. 2020 Jul 20;86(15). doi: 10.1128/AEM.00678-20.
4
Revealing the Membrane-Bound Catalytic Oxidation of NADH by the Drug Target Type-II NADH Dehydrogenase.揭示药物靶标Ⅱ型烟酰胺腺嘌呤二核苷酸脱氢酶对烟酰胺腺嘌呤二核苷酸的膜结合催化氧化作用。
Biochemistry. 2019 Oct 22;58(42):4272-4275. doi: 10.1021/acs.biochem.9b00752. Epub 2019 Oct 10.
5
Alternative Type II NAD(P)H Dehydrogenases in the Mitochondria of Protists and Fungi.原生生物和真菌线粒体中的另类II型烟酰胺腺嘌呤二核苷酸（磷酸）脱氢酶
Protist. 2019 Feb;170(1):21-37. doi: 10.1016/j.protis.2018.11.001. Epub 2018 Nov 10.
6
A single Gal4-like transcription factor activates the Crabtree effect in Komagataella phaffii.单一的 Gal4 样转录因子激活了 Komagataella phaffii 的 Crabtree 效应。
Nat Commun. 2018 Nov 21;9(1):4911. doi: 10.1038/s41467-018-07430-4.
7
Evaluation of a novel cloud-based software platform for structured experiment design and linked data analytics.新型基于云的结构化实验设计和关联数据分析软件平台的评估。
Sci Data. 2018 Oct 3;5:180195. doi: 10.1038/sdata.2018.195.
8
YAAM: Yeast Amino Acid Modifications Database.YAAM：酵母氨基酸修饰数据库。
Database (Oxford). 2018 Jan 1;2018. doi: 10.1093/database/bax099.
9
Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid.使用广谱 Cas9/gRNA 共表达质粒在克鲁维酵母和奥塔维亚酵母中进行基因组编辑。
FEMS Yeast Res. 2018 May 1;18(3). doi: 10.1093/femsyr/foy012.
10
Comparative genomics of biotechnologically important yeasts.具有重要生物技术意义的酵母的比较基因组学
Proc Natl Acad Sci U S A. 2016 Aug 30;113(35):9882-7. doi: 10.1073/pnas.1603941113. Epub 2016 Aug 17.