Brijwani Khushal, Vadlani Praveen V
Bioprocessing Laboratory, 201 Shellenberger Hall, Department of Grain Science and Industry, Kansas State University, Manhattan, KS 66506, USA.
Enzyme Res. 2011;2011:860134. doi: 10.4061/2011/860134. Epub 2011 Jun 15.
We investigated the effect of pretreatment on the physicochemical characteristics-crystallinity, bed porosity, and volumetric specific surface of soybean hulls and production of cellulolytic enzymes in solid-state fermentation of Trichoderma reesei and Aspergillus oryzae cultures. Mild acid and alkali and steam pretreatments significantly increased crystallinity and bed porosity without significant change inholocellulosic composition of substrate. Crystalline and porous steam-pretreated soybean hulls inoculated with T. reesei culture had 4 filter paper units (FPU)/g-ds, 0.6 IU/g-ds β-glucosidase, and 45 IU/g-ds endocellulase, whereas untreated hulls had 0.75 FPU/g-ds, 0.06 IU/g-ds β-glucosidase, and 7.29 IU/g-ds endocellulase enzyme activities. In A. oryzae steam-pretreated soybean hulls had 47.10 IU/g-ds endocellulase compared to 30.82 IU/g-ds in untreated soybean hulls. Generalized linear statistical model fitted to enzyme activity data showed that effects of physicochemical characteristics on enzymes production were both culture and enzyme specific. The paper shows a correlation between substrate physicochemical properties and enzyme production.
我们研究了预处理对大豆皮物理化学特性（结晶度、床层孔隙率和体积比表面积）的影响，以及里氏木霉和米曲霉固态发酵过程中纤维素酶的产生情况。温和的酸碱处理和蒸汽预处理显著提高了结晶度和床层孔隙率，而底物的全纤维素组成没有显著变化。接种里氏木霉培养物的经蒸汽预处理的结晶且多孔的大豆皮具有4个滤纸酶活力单位（FPU）/克干物质、0.6国际单位/克干物质的β-葡萄糖苷酶和45国际单位/克干物质的内切纤维素酶，而未处理的大豆皮具有0.75 FPU/克干物质、0.06国际单位/克干物质的β-葡萄糖苷酶和7.29国际单位/克干物质的内切纤维素酶活性。在米曲霉中，经蒸汽预处理的大豆皮具有47.10国际单位/克干物质的内切纤维素酶，而未处理的大豆皮为30.82国际单位/克干物质。拟合酶活性数据的广义线性统计模型表明，物理化学特性对酶产生的影响具有培养物和酶的特异性。本文显示了底物物理化学性质与酶产生之间的相关性。
