Cannon J G, Orencole S F, Fielding R A, Meydani M, Meydani S N, Fiatarone M A, Blumberg J B, Evans W J
United States Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts.
Am J Physiol. 1990 Dec;259(6 Pt 2):R1214-9. doi: 10.1152/ajpregu.1990.259.6.R1214.
Several host defense responses and metabolic reactions that occur during infection have been observed after exercise. We hypothesized that these reactions, known as the "acute phase response," contribute to the breakdown and clearance of damaged tissue after exercise. This hypothesis was tested with 21 male volunteers representing two ranges of age (22-29 and 55-74 yr), who ran downhill on an inclined treadmill to accentuate damaging eccentric muscular contractions. The subject groups were further divided in a double-blind placebo-controlled protocol, which examined the influence of 48 days of dietary vitamin E supplementation before the exercise. All subjects were monitored for 12 days after exercise for changes in circulating leukocytes, superoxide release from neutrophils, lipid peroxidation, and efflux of the intramuscular enzyme creatine kinase (CK) into the circulation. Among those receiving placebo, the less than 30-yr-old subjects responded to exercise with a significantly greater neutrophilia and higher plasma CK concentrations than the greater than 55-yr-old subjects. Dietary supplementation with vitamin E tended to eliminate the differences between the two age groups, primarily by increasing the responses of the greater than 55-yr-old subjects. At the time of peak concentrations in the plasma, CK correlated significantly with superoxide release from neutrophils. The association of enzyme efflux with neutrophil mobilization and function supports the concept that neutrophils are involved in the delayed increase in muscle membrane permeability after damaging exercise.
运动后已观察到感染期间发生的几种宿主防御反应和代谢反应。我们假设这些被称为“急性期反应”的反应有助于运动后受损组织的分解和清除。我们用21名男性志愿者对这一假设进行了测试，他们代表两个年龄范围（22 - 29岁和55 - 74岁），在倾斜的跑步机上向下跑，以加剧破坏性的离心肌肉收缩。受试者组在双盲安慰剂对照方案中进一步分组，该方案研究了运动前48天膳食补充维生素E的影响。运动后对所有受试者进行了12天的监测，以观察循环白细胞、中性粒细胞超氧化物释放、脂质过氧化以及肌肉内酶肌酸激酶（CK）向循环中的外流情况的变化。在接受安慰剂的受试者中，年龄小于30岁的受试者对运动的反应是中性粒细胞增多显著且血浆CK浓度高于年龄大于55岁的受试者。膳食补充维生素E倾向于消除两个年龄组之间的差异，主要是通过增加年龄大于55岁受试者的反应。在血浆浓度达到峰值时，CK与中性粒细胞的超氧化物释放显著相关。酶外流与中性粒细胞动员和功能的关联支持了中性粒细胞参与损伤性运动后肌肉膜通透性延迟增加的概念。
