Meydani M, Evans W J, Handelman G, Biddle L, Fielding R A, Meydani S N, Burrill J, Fiatarone M A, Blumberg J B, Cannon J G
Antioxidant Research Laboratory, Tufts University, Boston, Massachusetts 02111.
Am J Physiol. 1993 May;264(5 Pt 2):R992-8. doi: 10.1152/ajpregu.1993.264.5.R992.
The protective effect of vitamin E supplementation on exercise-induced oxidative damage was tested in 21 male volunteers. Nine young (22-29 yr) and 12 older (55-74 yr) sedentary male subjects participated in a double-blind protocol and received either 800 IU dl-alpha-tocopherol or a placebo daily. After 48 days, vitamin E supplementation significantly increased alpha-tocopherol in plasma and skeletal muscle. Subjects then performed a bout of eccentric exercise at 75% of their maximum heart rate by running down an inclined treadmill for 45 min. All vitamin E-supplemented subjects excreted less (P < 0.05) urinary thiobarbituric acid adducts after the exercise bout than placebo subjects at 12 days postexercise (35 and 18% above baseline in young and old supplemented groups, respectively, vs. 60 and 80% in young and old placebo groups, respectively). After exercise, the initial difference in alpha-tocopherol concentration of muscle between young placebo and vitamin E-supplemented groups was diminished and muscle lipid conjugated dienes tended to increase (P = 0.09) in placebo subjects. Placebo subjects had a significant decrease in major fatty acids of muscle biopsy taken immediately after exercise. When normalized for the hemoconcentration effects of exercise, the plasma concentration of vitamins E and C and uric acid showed no significant change. The alterations in fatty acid composition, vitamin E, and lipid conjugated dienes in muscle and in urinary lipid peroxides in controls after eccentric exercise are consistent with the concept that vitamin E provides protection against exercise-induced oxidative injury.
在21名男性志愿者身上测试了补充维生素E对运动诱导的氧化损伤的保护作用。9名年轻（22 - 29岁）和12名年长（55 - 74岁）久坐不动的男性受试者参与了一项双盲试验，每天接受800国际单位的右旋α-生育酚或安慰剂。48天后，补充维生素E显著提高了血浆和骨骼肌中的α-生育酚水平。然后，受试者以最大心率的75%在倾斜的跑步机上跑45分钟进行一次离心运动。运动后12天，所有补充维生素E的受试者尿中硫代巴比妥酸加合物的排泄量均低于安慰剂组（P < 0.05）（年轻和年长补充组分别比基线高35%和18%，而年轻和年长安慰剂组分别为60%和80%）。运动后，年轻安慰剂组和补充维生素E组之间肌肉中α-生育酚浓度的初始差异减小，安慰剂组肌肉脂质共轭二烯趋于增加（P = 0.09）。安慰剂组在运动后立即采集的肌肉活检主要脂肪酸显著减少。在对运动引起的血液浓缩效应进行校正后，血浆中维生素E、维生素C和尿酸的浓度没有显著变化。离心运动后对照组肌肉中脂肪酸组成、维生素E、脂质共轭二烯以及尿脂质过氧化物的变化与维生素E可预防运动诱导的氧化损伤这一概念相符。
