处于不同感染临床阶段的HIV-1血清阳性患者中的人巨细胞病毒血症。

Human cytomegalovirus viraemia in HIV-1-seropositive patients at various clinical stages of infection.

作者信息

Gerna G, Parea M, Percivalle E, Zipeto D, Silini E, Barbarini G, Milanesi G

机构信息

Virus Laboratory, Institute of Infectious Diseases, University of Pavia, IRCCS Policlinico S. Matteo, Italy.

出版信息

AIDS. 1990 Oct;4(10):1027-31. doi: 10.1097/00002030-199010000-00014.

DOI:10.1097/00002030-199010000-00014

PMID:2175616

Abstract

Eighty-two HIV-1-seropositive subjects were examined for the presence and quantification of human cytomegalovirus (HCMV) in peripheral blood polymorphonuclear leukocytes (PMNL) by polymerase chain reaction, culture and immunofluorescence in order to investigate the relationship between viraemia and immunosuppression. Patients were divided into three groups: (1) asymptomatic subjects with greater than 400 x 10(6)/l CD4 lymphocytes (n = 30); (2) asymptomatic subjects with less than 400 x 10(6)/l of CD4 lymphocytes and zidovudine (n = 20), and (3) AIDS-related complex (ARC)/AIDS patients on zidovudine (n = 32). Evidence of HCMV infection in circulating PMNL was found in 15 out of 29 ARC/AIDS patients examined (51.7%), whereas no infection was detected among the 50 asymptomatic HIV-1-seropositive subjects. HCMV-related symptoms were found only where the number of infected PMNL was greater than 50 per 2 x 10(5) cells.

摘要

通过聚合酶链反应、培养和免疫荧光法，对82名HIV-1血清反应阳性受试者外周血多形核白细胞（PMNL）中的人巨细胞病毒（HCMV）进行检测，以研究病毒血症与免疫抑制之间的关系。患者被分为三组：（1）CD4淋巴细胞大于400×10⁶/l的无症状受试者（n = 30）；（2）CD4淋巴细胞少于400×10⁶/l且接受齐多夫定治疗的无症状受试者（n = 20），以及（3）接受齐多夫定治疗的艾滋病相关综合征（ARC）/艾滋病患者（n = 32）。在29名接受检查的ARC/艾滋病患者中，有15名（51.7%）在循环PMNL中发现了HCMV感染的证据，而在50名无症状HIV-1血清反应阳性受试者中未检测到感染。仅在每2×10⁵个细胞中感染的PMNL数量大于50时才发现HCMV相关症状。