Tsuchida Y, Mochida Y, Kamii Y, Honna T, Saeki M, Hata J, Yokoyama T, Sasaki F, Nishiura M, Takeda K
Department of Surgery, National Children's Hospital, Tokyo, Japan.
J Pediatr Surg. 1990 Oct;25(10):1092-4. doi: 10.1016/0022-3468(90)90226-y.
Plasma total renin is a new, useful marker for nephroblastoma, but the conventional method for determining its level is sophisticated and requires large blood samples. To develop a simpler technique, a monoclonal antibody specific for both inactive and active renin (inactive + active = total) was raised, and a radioimmunoassay (RIA) system was established. This monoclonal antibody stains only the juxtaglomerular apparatus; values determined by this RIA did not change before and after activation. So far, the RIA system has been applied to 136 samples from 92 patients. Plasma total renin levels were also determined with the conventional method: samples were activated, then renin activity was assayed by measuring angiotensin I. The coefficient of the data obtained by these two different techniques was 0.921 (P less than .01). Plasma total renin levels in patients with nephroblastoma were significantly increased (546.5 +/- 297.8 pg/mL) over those in patients with neuroblastoma (218.6 +/- 46.5 pg/mL) and in controls (165.8 +/- 67.5 pg/mL, P less than .01). After removal of Wilms' tumors, the levels decreased to normal when sampled every 2 weeks. It was concluded that a newly developed RIA system is more useful in determining plasma total renin levels in patients with nephroblastoma.
血浆总肾素是肾母细胞瘤一种新的有用标志物，但测定其水平的传统方法复杂，且需要大量血液样本。为开发一种更简单的技术，制备了一种对无活性和活性肾素均特异的单克隆抗体（无活性+活性=总肾素），并建立了放射免疫分析（RIA）系统。这种单克隆抗体仅对球旁器染色；通过该RIA测定的值在激活前后无变化。到目前为止，该RIA系统已应用于92例患者的136份样本。还用传统方法测定了血浆总肾素水平：样本被激活，然后通过测量血管紧张素I来测定肾素活性。这两种不同技术所获数据的相关系数为0.921（P<0.01）。肾母细胞瘤患者的血浆总肾素水平显著高于神经母细胞瘤患者（218.6±46.5 pg/mL）和对照组（165.8±67.5 pg/mL，P<0.01）。切除威尔姆斯瘤后，每2周采样一次时，血浆总肾素水平降至正常。得出的结论是，新开发的RIA系统在测定肾母细胞瘤患者血浆总肾素水平方面更有用。
