Mochida Y, Tsuchida Y, Hata J, Komura M, Nishiura M
Department of Surgery, National Children's Hospital, Tokyo, Japan.
Tumour Biol. 1991;12(2):75-81. doi: 10.1159/000217691.
The determination of plasma total renin is useful not only as a tool to investigate the physiology of hypertension but also as a marker for Wilms' tumor. A radioimmunoassay (RIA) system to determine plasma total renin was newly developed using monoclonal antibodies specific for both inactive and active renin (inactive + active = total); in this study, an effort was made to confirm that this RIA system truly determines plasma total renin concentrations. First, it was found that this monoclonal antibody stains only the juxtaglomerular apparatus of the kidney. Second, the data determined by the RIA were compared with those obtained by the conventional enzymatic method: samples were activated, and renin activity was assayed by measuring angiotensin I. The coefficient of the data obtained by this RIA system and by the conventional method was 0.921 (p less than 0.01) based on all 89 samples, and 0.809 (p less than 0.01) based on 86 of the 89 samples whose values were less than 600 pg/ml by RIA. As a result of these studies, it was concluded that the newly developed RIA system does determine total renin levels in patients' plasma.
血浆总肾素的测定不仅是研究高血压生理学的一种工具，也是威尔姆斯瘤的一个标志物。一种用于测定血浆总肾素的放射免疫分析（RIA）系统被新开发出来，它使用了对无活性和活性肾素均具有特异性的单克隆抗体（无活性+活性=总肾素）；在本研究中，人们努力证实该RIA系统确实能测定血浆总肾素浓度。首先，发现这种单克隆抗体仅对肾脏的球旁器进行染色。其次，将RIA测定的数据与通过传统酶法获得的数据进行比较：将样本激活，并通过测量血管紧张素I来测定肾素活性。基于全部89个样本，该RIA系统和传统方法获得的数据的系数为0.921（p<0.01），基于89个样本中RIA值小于600 pg/ml的86个样本，该系数为0.809（p<0.01）。这些研究的结果是，得出结论认为新开发的RIA系统确实能测定患者血浆中的总肾素水平。
