Sugimoto K, Shionoiri H, Minamisawa K, Abe Y, Ueda S, Himeno H, Matsukawa T, Gotoh E, Ishii M
Second Department of Internal Medicine, Yokohama City University School of Medicine, Japan.
Am J Med Sci. 1991 Dec;302(6):342-6. doi: 10.1097/00000441-199112000-00003.
The authors evaluated the assay performances and clinical usefulness of a newly developed solid phase radioimmunoassay (RIA) for total renin concentration (TRC) in human plasma. The direct total renin RIA was performed by a sandwich technique with a pair of anti-human renin monoclonal antibodies. Renin activation with trypsin did not change TRC. The RIA showed satisfactory assay performances and demonstrated full compatibility with a direct RIA-kit for active renin concentration (ARC) in human plasma. The values of TRC were 105.3 +/- 8.6 pg/mL in normal subjects and 136.5 +/- 14.6 pg/mL in patients with essential hypertension. The values of TRC and the ratios of ARC to TRC were high in patients with renovascular hypertension and were low in patients with primary aldosteronism. Although the TRC value in diabetic patients was 134.4 +/- 14.8 pg/mL, the ratio of ARC to TRC was low. The RIA procedure was simple since prior purification or activation of renin was not required. These results suggest that the total renin RIA and its combined use with the active renin RIA may be helpful in understanding the renin-angiotensin system in human plasma.
作者评估了一种新开发的用于检测人血浆中总肾素浓度（TRC）的固相放射免疫分析（RIA）的分析性能和临床实用性。直接总肾素RIA采用夹心技术，使用一对抗人肾素单克隆抗体进行。用胰蛋白酶激活肾素不会改变TRC。该RIA显示出令人满意的分析性能，并证明与用于检测人血浆中活性肾素浓度（ARC）的直接RIA试剂盒完全兼容。正常受试者的TRC值为105.3±8.6 pg/mL，原发性高血压患者的TRC值为136.5±14.6 pg/mL。肾血管性高血压患者的TRC值和ARC与TRC的比值较高，原发性醛固酮增多症患者的TRC值和ARC与TRC的比值较低。尽管糖尿病患者的TRC值为134.4±14.8 pg/mL，但其ARC与TRC的比值较低。由于不需要事先对肾素进行纯化或激活，RIA程序很简单。这些结果表明，总肾素RIA及其与活性肾素RIA的联合使用可能有助于了解人血浆中的肾素-血管紧张素系统。
