• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

肾小球毛细血管对 HLA 类 I 抗体的体外和体内 miRNA 反应。

Glomerulocapillary miRNA response to HLA-class I antibody in vitro and in vivo.

机构信息

Institute for Transfusion Medicine, University Hospital Essen, University Duisburg-Essen, Essen, Germany.

Immune Evaluation Laboratory, Department of Surgery, Baylor College of Medicine, Houston, TX, USA.

出版信息

Sci Rep. 2017 Nov 6;7(1):14554. doi: 10.1038/s41598-017-14674-5.

DOI:10.1038/s41598-017-14674-5
PMID:29109529
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5673998/
Abstract

UNLABELLED

Changes in miRNA expression glomerular of capillaries during antibody-mediated rejection (ABMR) are poorly understood and could contribute to the deleterious inflammation and fibrosis of ABMR via suppression of target genes. A better understanding could lead to novel diagnostic tools and reveal novel therapeutic targets. We explored deregulated miRNAs in an glomeruloendothelial in vitro model of ABMR due to class I human leukocyte antigen (HLA) with and without complement activation. We studied a set of 16 promising candidate miRNAs in microdissected glomeruli a confirmation set of 20 human transplant biopsies (DSA+) compared to 10 matched controls without evidence for ABMR. Twelve out of these 16 glomerulocapillary miRNAs could successfully be confirmed as dysregulated in vivo with 10 upregulated (let-7c-5p, miR-28-3p, miR-30d-5p, miR-99b-5p, miR-125a-5p, miR-195-5p, miR-374b-3p, miR-484, miR-501-3p, miR-520e) and 2 downregulated (miR29b-3p, miR-885-5p) in DSA+ vs.

CONTROLS

A random forest analysis based on glomerular miRNAs identified 18/20 DSA+ and 8/10 controls correctly. This glomerulocapillary miRNA signature associated with HLA class I-DSA could improve our understanding of ABMR and be useful for diagnostic or therapeutic purposes.

摘要

未加标签

抗体介导的排斥反应（ABMR）期间肾小球毛细血管中 miRNA 表达的变化知之甚少，通过抑制靶基因，可能导致 ABMR 的有害炎症和纤维化。更好的理解可能导致新的诊断工具，并揭示新的治疗靶点。我们在体外肾小球内皮 ABMR 模型中探索了因 HLA 类 I 而失调的 miRNA，同时也有和没有补体激活。我们研究了一组 16 个有前途的候选 miRNA，在微切割肾小球中的一个确认组 20 个人移植活检（DSA+）与 10 个匹配的无 ABMR 证据的对照相比。这 16 个肾小球毛细血管 miRNA 中有 12 个可以成功地在体内被证实为失调，其中 10 个上调（let-7c-5p、miR-28-3p、miR-30d-5p、miR-99b-5p、miR-125a-5p、miR-195-5p、miR-374b-3p、miR-484、miR-501-3p、miR-520e）和 2 个下调（miR29b-3p、miR-885-5p）在 DSA+vs.对照组中。

对照

基于肾小球 miRNA 的随机森林分析正确地识别了 18/20 个 DSA+和 8/10 个对照。这个与 HLA 类 I-DSA 相关的肾小球毛细血管 miRNA 特征可以提高我们对 ABMR 的理解，并有助于诊断或治疗目的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a15/5673998/28ad48072071/41598_2017_14674_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a15/5673998/34d5e28c706b/41598_2017_14674_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a15/5673998/a146d026c46f/41598_2017_14674_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a15/5673998/da1ae2c0b658/41598_2017_14674_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a15/5673998/434479baad5e/41598_2017_14674_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a15/5673998/28ad48072071/41598_2017_14674_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a15/5673998/34d5e28c706b/41598_2017_14674_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a15/5673998/a146d026c46f/41598_2017_14674_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a15/5673998/da1ae2c0b658/41598_2017_14674_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a15/5673998/434479baad5e/41598_2017_14674_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a15/5673998/28ad48072071/41598_2017_14674_Fig5_HTML.jpg

相似文献

1
Glomerulocapillary miRNA response to HLA-class I antibody in vitro and in vivo.肾小球毛细血管对 HLA 类 I 抗体的体外和体内 miRNA 反应。
Sci Rep. 2017 Nov 6;7(1):14554. doi: 10.1038/s41598-017-14674-5.
2
Histological picture of antibody-mediated rejection without donor-specific anti-HLA antibodies: Clinical presentation and implications for outcome.抗体介导排斥反应而无供体特异性抗 HLA 抗体的组织学图像：临床表现和对结局的影响。
Am J Transplant. 2019 Mar;19(3):763-780. doi: 10.1111/ajt.15074. Epub 2018 Sep 28.
3
Identifying Subphenotypes of Antibody-Mediated Rejection in Kidney Transplants.鉴定肾移植中抗体介导排斥的亚表型。
Am J Transplant. 2016 Mar;16(3):908-20. doi: 10.1111/ajt.13551. Epub 2016 Jan 6.
4
Transcriptional Changes in Kidney Allografts with Histology of Antibody-Mediated Rejection without Anti-HLA Donor-Specific Antibodies.抗体介导的排斥反应组织学但无 HLA 供体特异性抗体的同种异体肾移植中的转录变化。
J Am Soc Nephrol. 2020 Sep;31(9):2168-2183. doi: 10.1681/ASN.2020030306. Epub 2020 Jul 8.
5
Chronic Active Antibody-Mediated Rejection Is Associated With the Upregulation of Interstitial But Not Glomerular Transcripts.慢性持续性抗体介导的排斥反应与间质而不是肾小球转录物的上调有关。
Front Immunol. 2021 Sep 20;12:729558. doi: 10.3389/fimmu.2021.729558. eCollection 2021.
6
Differential modulation of donor-specific antibodies after B-cell depleting therapies to cure chronic antibody mediated rejection.B细胞清除疗法治疗慢性抗体介导排斥反应后供体特异性抗体的差异调节
Transplantation. 2015 Jan;99(1):63-8. doi: 10.1097/TP.0000000000000285.
7
C3d-binding Donor-specific HLA Antibody Is Associated With a High Risk of Antibody-mediated Rejection and Graft Loss in Stable Kidney Transplant Recipients: A Single-center Cohort Study.C3d结合供者特异性HLA抗体与稳定肾移植受者抗体介导的排斥反应及移植物丢失高风险相关：一项单中心队列研究
Transplant Proc. 2018 Dec;50(10):3452-3459. doi: 10.1016/j.transproceed.2018.06.037. Epub 2018 Jul 2.
8
Integrative Omics Analysis Unravels Microvascular Inflammation-Related Pathways in Kidney Allograft Biopsies.整合组学分析揭示了肾移植活检中与微血管炎症相关的途径。
Front Immunol. 2021 Nov 2;12:738795. doi: 10.3389/fimmu.2021.738795. eCollection 2021.
9
Chronic Active Antibody-Mediated Rejection with Linear IgG Deposition on Glomerular Capillaries in a Kidney Transplant Recipient.肾移植受者中出现慢性活动性抗体介导的排斥反应，伴有肾小球毛细血管线性IgG沉积。
Nephron. 2020;144 Suppl 1:97-101. doi: 10.1159/000511322. Epub 2020 Nov 25.
10
MTUS1 and its targeting miRNAs in colorectal carcinoma: significant associations.MTUS1及其靶向的微小RNA在结直肠癌中的显著关联
Tumour Biol. 2016 May;37(5):6637-45. doi: 10.1007/s13277-015-4550-4. Epub 2015 Dec 7.

引用本文的文献

1
New insights into maladaptive vascular responses to donor specific HLA antibodies in organ transplantation.器官移植中对供体特异性HLA抗体的适应性血管反应的新见解。
Front Transplant. 2023 Apr 28;2:1146040. doi: 10.3389/frtra.2023.1146040. eCollection 2023.
2
Downregulation of and Expression in a Wistar Rat Diabetic Model during Diabetes Onset.糖尿病发病期间Wistar大鼠糖尿病模型中 和 表达的下调
Curr Diabetes Rev. 2025;21(5):e200624231125. doi: 10.2174/0115733998264880230919062657.
3
MicroRNAs as Potential Graft Rejection or Tolerance Biomarkers and Their Dilemma in Clinical Routines Behaving like Devilish, Angelic, or Frightening Elements.

本文引用的文献

1
The Banff 2015 Kidney Meeting Report: Current Challenges in Rejection Classification and Prospects for Adopting Molecular Pathology.《2015年班夫肾脏会议报告：排斥反应分类的当前挑战及采用分子病理学的前景》
Am J Transplant. 2017 Jan;17(1):28-41. doi: 10.1111/ajt.14107.
2
Regulatory non-coding RNA: new instruments in the orchestration of cell death.调控性非编码RNA：细胞死亡调控中的新工具
Cell Death Dis. 2016 Aug 11;7(8):e2333. doi: 10.1038/cddis.2016.210.
3
MicroRNAs: The Role in Autoimmune Inflammation.微小RNA：在自身免疫性炎症中的作用
微小RNA作为潜在的移植物排斥或耐受生物标志物及其在临床常规中的困境：表现得像恶魔、天使或令人恐惧的元素
Biomedicines. 2024 Jan 5;12(1):116. doi: 10.3390/biomedicines12010116.
4
The Transplant Bellwether: Endothelial Cells in Antibody-Mediated Rejection.移植风向标：抗体介导排斥反应中的内皮细胞。
J Immunol. 2023 Nov 1;211(9):1276-1285. doi: 10.4049/jimmunol.2300363.
5
The Role of Immune-Related miRNAs in the Pathology of Kidney Transplantation.免疫相关 miRNA 在肾移植病理学中的作用。
Biomolecules. 2021 Aug 12;11(8):1198. doi: 10.3390/biom11081198.
6
Enhancement of myogenic differentiation and inhibition of rhabdomyosarcoma progression by miR-28-3p and miR-193a-5p regulated by SNAIL.由SNAIL调控的miR-28-3p和miR-193a-5p增强成肌分化并抑制横纹肌肉瘤进展
Mol Ther Nucleic Acids. 2021 Apr 20;24:888-904. doi: 10.1016/j.omtn.2021.04.013. eCollection 2021 Jun 4.
7
Identifying Differentially Expressed MicroRNAs, Target Genes, and Key Pathways Deregulated in Patients with Liver Diseases.鉴定肝病患者中差异表达的 microRNAs、靶基因和关键失调通路。
Int J Mol Sci. 2020 Oct 6;21(19):7368. doi: 10.3390/ijms21197368.
8
Diagnostic, Prognostic, and Therapeutic Value of Non-Coding RNA Expression Profiles in Renal Transplantation.非编码 RNA 表达谱在肾移植中的诊断、预后及治疗价值
Diagnostics (Basel). 2020 Jan 22;10(2):60. doi: 10.3390/diagnostics10020060.
9
Expression of microRNA-99a-3p in Prostate Cancer Based on Bioinformatics Data and Meta-Analysis of a Literature Review of 965 Cases.基于生物信息学数据和对 965 例文献综述的荟萃分析评估前列腺癌中 microRNA-99a-3p 的表达。
Med Sci Monit. 2018 Jul 12;24:4807-4822. doi: 10.12659/MSM.908057.
Acta Naturae. 2016 Jan-Mar;8(1):21-33.
4
A Single Let-7 MicroRNA Bypasses LIN28-Mediated Repression.单个Let-7微小RNA绕过LIN28介导的抑制作用。
Cell Rep. 2015 Oct 13;13(2):260-6. doi: 10.1016/j.celrep.2015.08.086. Epub 2015 Oct 1.
5
Cdc42 is required for cytoskeletal support of endothelial cell adhesion during blood vessel formation in mice.在小鼠血管形成过程中，内皮细胞黏附的细胞骨架支持需要Cdc42。
Development. 2015 Sep 1;142(17):3058-70. doi: 10.1242/dev.125260. Epub 2015 Aug 7.
6
A role of uridylation pathway for blockade of let-7 microRNA biogenesis by Lin28B.尿苷酸化途径在Lin28B阻断let-7微小RNA生物合成过程中的作用。
Cancer Sci. 2015 Sep;106(9):1174-81. doi: 10.1111/cas.12721. Epub 2015 Jul 14.
7
NRP1 Regulates CDC42 Activation to Promote Filopodia Formation in Endothelial Tip Cells.神经纤毛蛋白1（NRP1）调节细胞分裂周期蛋白42（CDC42）的激活以促进内皮尖端细胞中丝状伪足的形成。
Cell Rep. 2015 Jun 16;11(10):1577-90. doi: 10.1016/j.celrep.2015.05.018. Epub 2015 Jun 4.
8
DIANA-miRPath v3.0: deciphering microRNA function with experimental support.DIANA-miRPath v3.0：借助实验支持解读微小RNA功能。
Nucleic Acids Res. 2015 Jul 1;43(W1):W460-6. doi: 10.1093/nar/gkv403. Epub 2015 May 14.
9
MicroRNAs in renal fibrosis.肾纤维化中的微小RNA
Front Physiol. 2015 Feb 20;6:50. doi: 10.3389/fphys.2015.00050. eCollection 2015.
10
Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of endothelial filopodia in zebrafish.Cdc42 通过 Fmnl3 驱动的内皮细胞丝状伪足组装介导 Bmp 诱导的血管发芽。
Dev Cell. 2015 Jan 12;32(1):109-22. doi: 10.1016/j.devcel.2014.11.024.