Choi Jin Kyeong, Kim Sang-Hyun
BK21 Plus KNU Biomedical Convergence Program, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
BK21 Plus KNU Biomedical Convergence Program, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
Food Chem Toxicol. 2014 Jun;68:135-41. doi: 10.1016/j.fct.2014.03.021. Epub 2014 Mar 25.
Galangin is a member of the flavonol class of flavonoids having anti-inflammatory and anti-oxidative potential. Previously we reported the inhibitory effect of galangin on the mast cell-mediated allergic inflammation. For incremental research, we investigated the effects of galangin on atopic dermatitis (AD)-like skin lesions and underlying mechanisms of action. We established an atopic dermatitis model in BALB/c mice by repeated local exposure of house dust mite (Dermatophagoides farinae) extract (DFE) and 2,4-dinitrochlorobenzene (DNCB) to the ears. Repeated alternative treatment of DFE/DNCB caused AD-like skin lesions. Topical application of galangin reduced AD symptoms based on ear thickness and histopathological analysis, in addition to serum IgE and IgG2a levels. Galangin inhibited mast cell infiltration into the ear and serum histamine level. Galangin suppressed DFE/DNCB-induced expression of interleukin (IL)-4, IL-5, IL-13, IL-31, IL-32, and interferon (IFN)-γ in the ear tissue. To define the underlying mechanisms of action, tumor necrosis factor-α/IFN-γ-activated human keratinocytes (HaCaT) model was used. Galangin significantly inhibited the expression of cytokines and chemokine by the down-regulation of nuclear factor-κB and mitogen-activated protein kinases in HaCaT cells. Taken together, the results demonstrate that galangin inhibited AD-like symptoms, suggesting that galangin might be a candidate for the treatment of AD.
高良姜素是具有抗炎和抗氧化潜力的黄酮醇类黄酮的成员。此前我们报道过高良姜素对肥大细胞介导的过敏性炎症的抑制作用。为了进一步研究，我们调查了高良姜素对特应性皮炎（AD）样皮肤损伤及其潜在作用机制的影响。我们通过将屋尘螨（粉尘螨）提取物（DFE）和2,4-二硝基氯苯（DNCB）反复局部涂抹于BALB/c小鼠耳部，建立了特应性皮炎模型。DFE/DNCB的反复交替处理导致了AD样皮肤损伤。除了血清IgE和IgG2a水平外，局部应用高良姜素还根据耳厚度和组织病理学分析减轻了AD症状。高良姜素抑制肥大细胞浸润到耳部以及血清组胺水平。高良姜素抑制了DFE/DNCB诱导的耳部组织中白细胞介素（IL）-4、IL-5、IL-13、IL-31、IL-32和干扰素（IFN）-γ的表达。为了确定潜在的作用机制，使用了肿瘤坏死因子-α/IFN-γ激活的人角质形成细胞（HaCaT）模型。高良姜素通过下调HaCaT细胞中的核因子-κB和丝裂原活化蛋白激酶，显著抑制了细胞因子和趋化因子的表达。综上所述，结果表明高良姜素抑制了AD样症状，提示高良姜素可能是治疗AD的候选药物。
