Choi Eun-Ju, Iwasa Masahiro, Han Kwon-Il, Kim Wan-Jae, Tang Yujiao, Han Weon Cheol, Kim Eun-Kyung, Park Zee-Yong
1Division of Sport Science, College of Science and Technology, Konkuk University, Chungju, Chungbuk, 27478 Korea.
R&D Center, Korea BeRM Co., Ltd., Seoul, 06163 Korea.
Food Sci Biotechnol. 2016 Aug 31;25(4):1087-1093. doi: 10.1007/s10068-016-0175-7. eCollection 2016.
Here, the effects of heat-killed Enterococcus faecalis EF-2001 (EF-2001) on atopic eczema (AE) were assessed. An AE model was established in vivo by repetitious topical exposure to 1-chloro-2,4-dinitrobenzene (CDNB) and dermatophagoidesfarinae extract (DFE) via application on each ear. Mice were administered EF-2001 orally for 4 weeks, dermal and epidermal ear thickness, mast cell infiltration of the ear tissue, and serum IgE and IgG2a levels were evaluated. Moreover, pathogenic cytokines levels of the ears, splenocytes, and cervical lymph nodes were determined. EF-2001 reduced AE symptoms grounded in the ear thickness, histopathological analysis, and serum IgE levels. Furthermore, EF-2001 attenuated mast cell infiltration in the ears and CDNB/DFE-induced various pathogenic cytokines levels of the ears, splenocytes and cervical lymph nodes. Thus, our data suggested that EF-2001 may have potential medicinal applications in the treatment of AE through its immunomodulatory properties.
在此，评估了热灭活粪肠球菌EF - 2001（EF - 2001）对特应性皮炎（AE）的影响。通过在每只耳朵上反复局部涂抹1 - 氯 - 2,4 - 二硝基苯（CDNB）和粉尘螨提取物（DFE）在体内建立AE模型。给小鼠口服EF - 2001持续4周，评估耳部皮肤和表皮厚度、耳部组织中的肥大细胞浸润以及血清IgE和IgG2a水平。此外，还测定了耳部、脾细胞和颈部淋巴结中致病性细胞因子的水平。基于耳部厚度、组织病理学分析和血清IgE水平，EF - 2001减轻了AE症状。此外，EF - 2001减少了耳部的肥大细胞浸润以及CDNB/DFE诱导的耳部、脾细胞和颈部淋巴结中各种致病性细胞因子的水平。因此，我们的数据表明EF - 2001可能通过其免疫调节特性在AE治疗中具有潜在的药用价值。
