Department of Botany, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India.
Acta Trop. 2011 Oct-Nov;120(1-2):115-8. doi: 10.1016/j.actatropica.2011.07.001. Epub 2011 Jul 7.
The vector-borne diseases caused by mosquitoes are one of the major health problems in many countries especially in tropical and sub-tropical countries. The resistance of mosquitoes to synthetic chemicals and environmental toxicity created by the chemicals raised the demand for finding of alternate natural molecules that control mosquito. In the present study, a crystalline compound methyl-p-hydroxybenzoate was isolated from the methanol extract of Vitex trifolia leaves and it was identified by (1)H and (13)C NMR and single crystal X-ray diffractometer. The larvicidal potential of the isolated compound was evaluated against early 4th instar larvae of Culex quinquefasciatus and Aedes aegypti. The compound exhibited 100% larval mortality of both the mosquitoes at 20 ppm with LC(50) values of 5.77 and 4.74 ppm against C. quinquefasciatus and A. aegypti, respectively. The methyl-p-hydroxybenzoate, which is reported for the first time to our best of knowledge from V. trifolia can be better explored for the control of mosquito population.
由蚊子传播的疾病是许多国家，特别是热带和亚热带国家的主要卫生问题之一。蚊子对合成化学品的抗药性以及化学品造成的环境毒性增加了寻找控制蚊子的替代天然分子的需求。在本研究中，从牡荆叶甲醇提取物中分离出一种结晶化合物对羟基苯甲酸甲酯，并通过（1）H 和（13）C NMR 以及单晶 X 射线衍射仪对其进行了鉴定。对分离得到的化合物对 4 龄初期的致倦库蚊和埃及伊蚊幼虫进行了杀幼虫活性评价。该化合物在 20 ppm 时对两种蚊子的幼虫死亡率均为 100%，对致倦库蚊和埃及伊蚊的 LC50 值分别为 5.77 和 4.74 ppm。对羟基苯甲酸甲酯是首次从牡荆叶中分离得到，据我们所知，它可以更好地用于控制蚊子种群。
