Baskar Kannan, Chinnasamy Ragavendran, Pandy Karthika, Venkatesan Manigandan, Sebastian Prakash Joy, Subban Murugesan, Thomas Adelina, Kweka Eliningaya J, Devarajan Natarajan
Natural Drug Research Laboratory, Department of Biotechnology, School of Biosciences, Periyar University, Salem 636 011, Tamil Nadu, India.
Biomedical Zebrafish Laboratory, Department of Medical Biotechnology, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam 603 103, Tamil Nadu, India.
Heliyon. 2020 Oct 28;6(10):e05331. doi: 10.1016/j.heliyon.2020.e05331. eCollection 2020 Oct.
Mosquitoes biolarvicides remain the most important method for mosquito control. The previous studies have shown -expressed larvicidal properties against mosquito species. The present study evaluated larvicidal and histopathological effect of an endophytic fungus isolated from theCactus stem ( Mill)
The molecular identification of isolated was done by PCR amplification (5.8s rDNA) using a universal primer (ITS-1 and ITS-2). The secondary metabolites of was tested for larvicidal activity against and . Larvicidal bioassay of different concentrations (- 100, 300, 500, 800 and 1000 μg/mL) isolated extracts were done according to the modified protocol. Each test included a set of control groups (i.e. DMSO and distilled water). The lethal concentrations (LC and LC) were calculated by probit analysis. Experimental monitoring duration was 48 h.
The ethyl acetate extract from fungus resulted - excellent mosquitocidal effect against and mosquitoes, with least LC and LC values. -After 48 h, the expressed better results (LC = 29.10, 18.69, 16.76, 36.78 μg/mL and the LC = 45.59, 27.66, 27.50, 54.00 μg/mL) followed by (LC = 3.23, 24.99, 11.24, 10.95 μg/mL and the LC = 8.37, 8.29, 21.36, 20.28 μg/mL). The biochemical level of mycelium extract on both larvae was measured and the results shown a dose dependent activity on the level of AchE, α- and β-carboxylesterase assay. Gas Chromatography and Mass Spectroscopy (GC-MS) profile of extract reflected three compounds i.e. preg-4-en-3-one, 17. α-hydroxy-17. β-cyano- (7.39%), trans-3-undecene-1,5-diyne (45.77%) and pentane, 1,1,1,5-tetrachloro- (32.16%) which which might had attributed to larvae mortality.
The findings of - present study shows that the use of endophytic fungal metabolites for control of dengue and filariasis vectors is promising and needs a semifield and small scale filed trials.
蚊虫生物杀幼虫剂仍然是控制蚊虫的最重要方法。先前的研究已经表明[某种物质]对蚊虫种类具有杀幼虫特性。本研究评估了从仙人掌茎（[仙人掌学名]）中分离出的一种内生真菌的杀幼虫和组织病理学效应。
使用通用引物（ITS - 1和ITS - 2）通过PCR扩增（5.8s rDNA）对分离出的[真菌名称]进行分子鉴定。测试了[真菌名称]的次生代谢产物对[蚊虫1名称]和[蚊虫2名称]的杀幼虫活性。根据改良方案对不同浓度（- 100、300、500、800和1000μg/mL）的分离提取物进行杀幼虫生物测定。每个测试都包括一组对照组（即二甲基亚砜和蒸馏水）。通过概率分析计算致死浓度（LC50和LC90）。实验监测持续时间为48小时。
[真菌名称]真菌的乙酸乙酯提取物对[蚊虫1名称]和[蚊虫2名称]蚊虫产生了极佳的杀蚊效果，LC50和LC90值最低。48小时后，[提取物1名称]表现出更好的结果（LC50 = 29.10、18.69、16.76、36.78μg/mL，LC90 = 45.59、27.66、27.
