IRF in Microdentistry, Via dell'Ariento 4, 50123 Florence, Italy.
Arch Oral Biol. 2011 Dec;56(12):1494-8. doi: 10.1016/j.archoralbio.2011.06.012. Epub 2011 Jul 20.
The aim of this study is to investigate the relationship between a vitamin D receptor polymorphism and the diagnosis of periodontal disease in non-smoker Italian patients with aggressive and chronic periodontitis.
DNA was obtained from the internal cheek mucosa of 115 patients with chronic periodontitis, 58 with aggressive periodontitis and 65 healthy controls. Allelic discrimination was performed using TaqMan SNP Genotyping Assays. Genotype and allele frequencies were calculated.
Comparisons between diseased patients and healthy controls showed significant differences. Moreover, calculating the odds ratio, individuals with the TT genotype, was more susceptible than individuals with tt to chronic periodontitis and individuals with Tt to aggressive periodontitis. Interestingly, the dominant model (TT + Tt vs. tt) was applicable to chronic periodontitis, whilst for aggressive periodontitis the recessive model (TT vs. Tt + tt) gave the highest odds ratio.
These data indicated that VDR TaqI polymorphism is differentially associated with development of chronic periodontitis and aggressive periodontitis in Italian population. The study of VDR polymorphisms may therefore be essential for the prevention of periodontitis and for a pre-treatment periodontal and/or for implant assessment. Moreover VDR TaqI polymorphism could be useful to discriminate between aggressive and chronic forms of periodontal disease.
本研究旨在探讨维生素 D 受体多态性与非吸烟意大利侵袭性和慢性牙周炎患者牙周病诊断之间的关系。
从 115 例慢性牙周炎患者、58 例侵袭性牙周炎患者和 65 例健康对照者的内颊黏膜中提取 DNA。采用 TaqMan SNP 基因分型检测进行等位基因鉴别。计算基因型和等位基因频率。
患病患者与健康对照组之间的比较显示出显著差异。此外，通过计算比值比，TT 基因型个体比 tt 基因型个体更易患慢性牙周炎，Tt 基因型个体比 tt 基因型个体更易患侵袭性牙周炎。有趣的是，显性模型（TT+Tt 与 tt）适用于慢性牙周炎，而对于侵袭性牙周炎，隐性模型（TT 与 Tt+tt）给出了最高的比值比。
这些数据表明，VDR TaqI 多态性与意大利人群慢性牙周炎和侵袭性牙周炎的发生存在差异相关性。因此，研究 VDR 多态性对于预防牙周炎以及牙周和/或种植前评估可能至关重要。此外，VDR TaqI 多态性可用于区分侵袭性和慢性牙周病。
