Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Key Lab of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Sci Rep. 2017 Aug 29;7(1):9587. doi: 10.1038/s41598-017-10191-7.
The formate pathway and NADH pathway as two common hydrogen-producing metabolic pathways have been well characterized to understand and improve biohydrogen production. These two pathways have been thought to be separate and have been independently investigated. However, in this study, perturbation of genes (hycA, fdhF, fhlA, ldhA, nuoB, hybO, fdh1, narP, and ppk) in Enterobacter aerogenes related to the formate pathway or NADH pathway revealed that these two pathways affected each other. Further metabolic analysis suggested that a linear relationship existed between the relative change of hydrogen yield in the formate pathway or NADH pathway and the relative change of NADH yield or ATP yield. Thus, this finding provides new insight into the role of cellular reducing power and energy level in the hydrogen metabolism. It also establishes a rationale for improving hydrogen production from a global perspective.
甲酸途径和 NADH 途径作为两种常见的产氢代谢途径，已经得到了很好的研究，以帮助我们理解和提高生物制氢的效率。这两种途径被认为是相互独立的，并分别进行了研究。然而，在本研究中，通过对与甲酸途径或 NADH 途径相关的基因（hycA、fdhF、fhlA、ldhA、nuoB、hybO、fdh1、narP 和 ppk）的基因敲除，发现这两种途径会相互影响。进一步的代谢分析表明，在甲酸途径或 NADH 途径中，氢气产量的相对变化与 NADH 产量或 ATP 产量的相对变化之间存在线性关系。因此，这一发现为细胞还原力和能量水平在氢代谢中的作用提供了新的见解。它也为从全局角度提高氢气产量提供了理论依据。
