Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Reprod Biomed Online. 2011 Sep;23(3):355-60. doi: 10.1016/j.rbmo.2011.05.005. Epub 2011 May 18.
Genetic factors play an important role in women's fertility and embryonic development and may also contribute to the efficacy of assisted reproduction techniques. The aim of this study was to investigate the effect of His447His and Pro12Ala peroxisome proliferator-activated receptor γ (PPARγ) gene polymorphisms on oocytes and fertilization in women undergoing IVF. Follicular fluid and blood samples were obtained from 98 IVF patients referred to Tabriz Alzahra Hospital. Samples were analysed for fatty acid content by gas-liquid chromatography and for polymorphisms of the PPARγ gene using polymerase chain reaction-restriction fragment length polymorphism-based methods. Multiple regression analyses were used to test the independence of associations between the number of mature and fertilized oocytes as outcome variables and the polymorphisms of PPARγ gene. For both polymorphisms, fertilization ratio was significantly (P<0.05) higher in carriers of the rare alleles than homozygous wild-type genotypes. The associations of His447His (P=0.003) and Pro12Ala (P=0.015) polymorphisms remained statistically significant in the multiple regression analyses. This study suggests that the two common gene polymorphisms of PPARγ may improve fertilization in vitro and, thus possibly, female fertility.
遗传因素在女性生育和胚胎发育中起着重要作用，也可能影响辅助生殖技术的效果。本研究旨在探讨过氧化物酶体增殖物激活受体 γ（PPARγ）基因 His447His 和 Pro12Ala 多态性对接受体外受精（IVF）治疗的女性卵母细胞和受精的影响。从 98 名转诊至大不里士阿扎赫拉医院的 IVF 患者中获取卵泡液和血液样本。通过气相色谱法分析脂肪酸含量，通过聚合酶链反应-限制性片段长度多态性方法分析 PPARγ 基因的多态性。采用多元回归分析检验 PPARγ 基因多态性与成熟和受精卵母细胞数量作为结局变量之间的关联的独立性。对于这两种多态性，稀有等位基因携带者的受精率明显（P<0.05）高于纯合野生型基因型。His447His（P=0.003）和 Pro12Ala（P=0.015）多态性在多元回归分析中仍然具有统计学意义。本研究表明，PPARγ 的两种常见基因多态性可能改善体外受精的受精能力，从而可能提高女性生育能力。
