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A431细胞细胞膜中磷酸肌醇激酶活性与蛋白质酪氨酸磷酸化之间的关系。

Relationship between phosphoinositide kinase activities and protein tyrosine phosphorylation in plasma membranes from A431 cells.

作者信息

Payrastre B, Plantavid M, Breton M, Chambaz E, Chap H

机构信息

INSERM U326, Hôpital Purpan, Toulouse, France.

出版信息

Biochem J. 1990 Dec 15;272(3):665-70. doi: 10.1042/bj2720665.

DOI:10.1042/bj2720665
PMID:2176471
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1149760/
Abstract

Production of PtdIns(4)P and PtdIns(4,5)P2 by plasma-membrane preparations from A431 cells was selectively stimulated in a dose-dependent manner by epidermal growth factor (EGF) in the presence of Na3VO4. Na3VO4 itself mimicked this effect, which was overcome after treatment by a specific phosphotyrosyl phosphatase isolated from A431 cells. PtdIns and PtdIns(4)P kinase activities were present in phosphotyrosyl-proteins isolated from EGF- and/or Na3VO4-stimulated A431 cells by immunoaffinity using an anti-phosphotyrosine antibody. These data suggest for the first time an EGF-dependent regulation of PtdIns 4-kinase and PtdIns(4)P 5-kinase activities by a mechanism involving a tyrosine-phosphorylation process.

摘要

在存在偏钒酸钠（Na3VO4）的情况下，表皮生长因子（EGF）以剂量依赖性方式选择性刺激A431细胞的质膜制剂产生磷脂酰肌醇-4-磷酸（PtdIns(4)P）和磷脂酰肌醇-4,5-二磷酸（PtdIns(4,5)P2）。Na3VO4本身模拟了这种效应，而从A431细胞中分离出的一种特异性磷酸酪氨酸磷酸酶处理后这种效应被消除。通过使用抗磷酸酪氨酸抗体进行免疫亲和，从EGF和/或Na3VO4刺激的A431细胞中分离出的磷酸酪氨酸蛋白中存在磷脂酰肌醇和磷脂酰肌醇-4-磷酸激酶活性。这些数据首次表明，通过涉及酪氨酸磷酸化过程的机制，EGF对磷脂酰肌醇4-激酶和磷脂酰肌醇-4-磷酸5-激酶活性具有依赖性调节作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b83/1149760/23f2a8a7b9e3/biochemj00169-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b83/1149760/23f2a8a7b9e3/biochemj00169-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b83/1149760/23f2a8a7b9e3/biochemj00169-0108-a.jpg

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