Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 598401, USA.
J Bacteriol. 2011 Sep;193(18):4993-5. doi: 10.1128/JB.05279-11. Epub 2011 Jul 15.
Transformation frequencies of a mariner-based transposon system in Rickettsia rickettsii were determined using a plaque assay system for enumeration and isolation of mutants. Sequence analysis of insertion sites in both R. rickettsii and R. prowazekii indicated that insertions were random. Transposon mutagenesis provides a useful tool for rickettsial research.
采用噬菌斑测定系统进行计数和分离突变体，测定了 mariner 转座子系统在立克次氏体中的转化频率。对立氏立克次体和普氏立克次体中插入位点的序列分析表明，插入是随机的。转座子诱变为立克次体研究提供了有用的工具。
