利用共培养的热纤梭菌和产丁醇梭菌 N1-4 从结晶纤维素生产丁醇。
Butanol production from crystalline cellulose by cocultured Clostridium thermocellum and Clostridium saccharoperbutylacetonicum N1-4.
机构信息
Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Setagaya-ku, Tokyo, Japan.
出版信息
Appl Environ Microbiol. 2011 Sep;77(18):6470-5. doi: 10.1128/AEM.00706-11. Epub 2011 Jul 15.
Abstract
We investigated butanol production from crystalline cellulose by cocultured cellulolytic Clostridium thermocellum and the butanol-producing strain, Clostridium saccharoperbutylacetonicum (strain N1-4). Butanol was produced from Avicel cellulose after it was incubated with C. thermocellum for at least 24 h at 60°C before the addition of strain N1-4. Butanol produced by strain N1-4 on 4% Avicel cellulose peaked (7.9 g/liter) after 9 days of incubation at 30°C, and acetone was undetectable in this coculture system. Less butanol was produced by cocultured Clostridium acetobutylicum and Clostridium beijerinckii than by strain N1-4, indicating that strain N1-4 was the optimal strain for producing butanol from crystalline cellulose in this coculture system.
摘要
我们研究了共培养纤维素分解梭菌和产丁醇菌株丙酮丁醇梭菌（N1-4 株）从结晶纤维素生产丁醇的情况。在向 N1-4 株中添加之前，用 C. thermocellum 将微晶纤维素孵育至少 24 小时，然后从 Avicel 纤维素中生产丁醇。N1-4 株在 30°C 下孵育 9 天后，在 4%的 Avicel 纤维素上生产的丁醇达到峰值（7.9 克/升），在该共培养体系中检测不到丙酮。与 N1-4 株相比，共培养的丙酮丁醇梭菌和拜氏梭菌产生的丁醇较少，表明 N1-4 株是该共培养体系中从结晶纤维素生产丁醇的最佳菌株。
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