Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
Department of Forest Science, Faculty of Regional Environment Science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
Bioresour Technol. 2015 Jun;186:325-328. doi: 10.1016/j.biortech.2015.03.061. Epub 2015 Mar 19.
The co-culture of cellulolytic Clostridium thermocellum NBRC 103400 and butanol-producing Clostridium saccharoperbutylacetonicum strain N1-4 produced 5.5 g/L of butanol from 40 g/L of delignified rice straw pretreated with 1% (wt/vol) NaOH. The addition of cellulase (100 U/g biomass) in a co-culture system significantly increased butanol production to 6.9 g/L using 40 g/L of delignified rice straw. Compared to the control, this increase in butanol production was attributed to the enhancement of exoglucanase activity on lignocellulose degradation in experimental samples. The results showed that the co-culture system in conjunction with enhanced exoglucanase activity resulted in cost-effective butanol production from delignified rice straw.
产丁醇梭菌（Clostridium saccharoperbutylacetonicum）N1-4 与纤维素分解梭菌（Clostridium thermocellum）NBRC 103400 的共培养物从用 1%（wt/vol）NaOH 预处理过的 40 g/L 去木质化稻秆中生产了 5.5 g/L 的丁醇。在共培养系统中添加纤维素酶（100 U/g 生物质）可显著提高丁醇产量，使用 40 g/L 的去木质化稻秆可生产 6.9 g/L 的丁醇。与对照相比，这种丁醇产量的增加归因于实验样品中木质纤维素降解的外切葡聚糖酶活性增强。结果表明，共培养系统与增强的外切葡聚糖酶活性相结合，可从去木质化稻秆中生产出具有成本效益的丁醇。
