Hjerpe A, Lindh E, Bistoletti P, George J, Groff D
Department of Pathology II, Karolinska Institute, Huddinge University Hospital Sweden.
Anal Quant Cytol Histol. 1990 Oct;12(5):299-305.
The use of Cytobrush samples for the detection and typing of cervical human papillomavirus (HPV) infections was analyzed. The average yield of squamous cells from Cytobrush samples was on the order of 10(6) cells when the sample was collected in a lytic collection buffer, which was approximately double the content of (1) samples collected in ethanol and (2) an average biopsy specimen. The material obtained could be used for sensitive detection and typing of HPV infections using, respectively, a nonradioactive dot-blot method and the Southern blot procedure performed with subgenomic probes, which permitted a simple interpretation even in cases of mixed infections. A sample containing at least 500,000 viral copies was required for the detection and typing. At this level of sensitivity, the frequencies of HPV obtained in different risk groups varied from 6% (in "healthy" young women) to 53% (in women with abnormal cytologic findings in simultaneous smears). The noninvasive nature of the sampling procedure and the relative simplicity of the test should allow this method to be applied in large-scale studies.
分析了使用细胞刷样本检测宫颈人乳头瘤病毒（HPV）感染及进行分型的情况。当样本收集于裂解收集缓冲液中时，细胞刷样本中鳞状细胞的平均产量约为10⁶个细胞，这大约是（1）收集于乙醇中的样本以及（2）平均活检标本细胞含量的两倍。所获得的材料可分别用于使用非放射性斑点印迹法和用亚基因组探针进行的Southern印迹程序对HPV感染进行灵敏检测和分型，即使在混合感染的情况下也能进行简单解读。检测和分型需要一个至少包含500,000个病毒拷贝的样本。在这种灵敏度水平下，不同风险组中HPV的检出率从6%（“健康”年轻女性）到53%（同时涂片细胞学检查异常的女性）不等。采样程序的非侵入性以及检测相对简单，应使该方法能够应用于大规模研究。
