Lindh E, Chua K L, Kataoka A, Bistoletti P, Groff D, Hjerpe A
Dept. of Medical Microbiology, Lund University, Malmö General Hospital, Sweden.
APMIS. 1992 Apr;100(4):301-8. doi: 10.1111/j.1699-0463.1992.tb00876.x.
A previously presented method for the detection and typing of human papillomavirus (HPV) DNA has been modified for the simultaneous analysis of HPV types 6, 11, 16, 18, 31, 33 and 35. The method has two steps, where a dot blot test is used to exclude cases that do not hybridize with HPV-DNA. The remaining cases are then analysed by a Southern blot procedure, using a mixture of subgenomic probes for the simple and accurate analysis of HPV types. When the procedure was used for the analysis of clinical samples, patient groups at varying risk were found to differ with regard to the prevalence of HPV infections. Thus, the virus was detected in only 8.8% of otherwise healthy young women - i.e. women without clinical signs of HPV-related disease - as compared to 48% of women who also had cervical intraepithelial neoplasia (CIN). A similarly high prevalence was found in patients in whom CIN persisted (42%) as compared to those in whom the morphological lesion regressed to normal (8%).
一种先前提出的用于检测人乳头瘤病毒（HPV）DNA及分型的方法已被改进，可同时分析HPV 6、11、16、18、31、33和35型。该方法有两个步骤，首先用斑点印迹试验排除与HPV-DNA不杂交的病例。然后，对剩余病例采用Southern印迹法进行分析，使用亚基因组探针混合物以简单准确地分析HPV类型。当该方法用于临床样本分析时，发现不同风险的患者组在HPV感染率方面存在差异。因此，在无其他异常的健康年轻女性中，即无HPV相关疾病临床体征的女性中，仅8.8%检测到该病毒，而在同时患有宫颈上皮内瘤变（CIN）的女性中这一比例为48%。与形态学病变恢复正常的患者（8%）相比，CIN持续存在的患者中也发现了类似的高感染率（42%）。
