Centre d'Immunologie de Marseille-Luminy, Université Aix-Marseille, Campus de Luminy, Marseille, France.
Nat Struct Mol Biol. 2011 Jul 17;18(8):956-63. doi: 10.1038/nsmb.2085.
Recent work has shown that RNA polymerase (Pol) II can be recruited to and transcribe distal regulatory regions. Here we analyzed transcription initiation and elongation through genome-wide localization of Pol II, general transcription factors (GTFs) and active chromatin in developing T cells. We show that Pol II and GTFs are recruited to known T cell-specific enhancers. We extend this observation to many new putative enhancers, a majority of which can be transcribed with or without polyadenylation. Importantly, we also identify genomic features called transcriptional initiation platforms (TIPs) that are characterized by large areas of Pol II and GTF recruitment at promoters, intergenic and intragenic regions. TIPs show variable widths (0.4-10 kb) and correlate with high CpG content and increased tissue specificity at promoters. Finally, we also report differential recruitment of TFIID and other GTFs at promoters and enhancers. Overall, we propose that TIPs represent important new regulatory hallmarks of the genome.
最近的研究表明，RNA 聚合酶（Pol）II 可以被募集到并转录远端调控区域。在这里，我们通过 Pol II、通用转录因子（GTFs）和活跃染色质在发育中的 T 细胞中的全基因组定位来分析转录起始和延伸。我们表明，Pol II 和 GTFs 被募集到已知的 T 细胞特异性增强子上。我们将这一观察结果扩展到许多新的假定增强子，其中大多数可以在有或没有多聚腺苷酸化的情况下进行转录。重要的是，我们还鉴定了称为转录起始平台（TIPs）的基因组特征，其特征是在启动子、基因间和基因内区域有大量的 Pol II 和 GTF 募集。TIPs 显示出可变的宽度（0.4-10 kb），并与启动子处的高 CpG 含量和增加的组织特异性相关。最后，我们还报告了 TFIID 和其他 GTFs 在启动子和增强子上的差异募集。总的来说，我们提出 TIPs 代表了基因组的重要新调控特征。
