Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, PR China.
Electrophoresis. 2011 Aug;32(16):2099-106. doi: 10.1002/elps.201100215. Epub 2011 Jul 18.
Using magnetite/silica/poly(methacrylic acid-co-ethylene glycol dimethacrylate) (Fe(3)O(4)/SiO(2)/poly(MAA-co-EDMA)) magnetic microspheres, a rapid and high-throughput magnetic solid-phase extraction coupled with capillary zone electrophoresis (MSPE-CZE) method was developed for the determination of illegal drugs (ketamine, amphetamines, opiates, and metabolites). The MSPE of target analytes could be completed within 2 min, and the eight target analytes could be baseline separated within 15 min by CZE with 30 mM phosphate buffer solution (PBS, pH 2.0) containing 15% v/v ACN as background electrolyte. Furthermore, hydrodynamic injection with field-amplified sample stacking (FASS) was employed to enhance the sensitivity of this MSPE-CZE method. Under such optimal conditions, the limits of detection for the eight target analytes ranged from 0.015 to 0.105 μg/mL. The application feasibility of MSPE-CZE in illegal drugs monitoring was demonstrated by analyzing urine samples, and the recoveries of target drugs for the spiked sample ranging from 85.4 to 110.1%. The method reproducibility was tested by evaluating the intra- and interday precisions, and relative standard deviations of <10.3 and 12.4%, respectively, were obtained. To increase throughput of the analysis, a home-made MSPE array that has potential application to the treatment of 96 samples simultaneously was used.
使用磁铁矿/二氧化硅/聚（甲基丙烯酸-co-乙二醇二甲基丙烯酸酯）（Fe3O4/SiO2/聚（MAA-co-EDMA））磁性微球，建立了一种快速高通量的磁固相萃取结合毛细管区带电泳（MSPE-CZE）方法，用于测定非法药物（氯胺酮、苯丙胺类、阿片类药物及其代谢物）。目标分析物的 MSPE 可在 2 分钟内完成，通过 CZE 用 30 mM 磷酸盐缓冲液（PBS，pH 2.0）作为背景电解质，其中含有 15%v/v ACN，可在 15 分钟内实现 8 种目标分析物的基线分离。此外，还采用了场放大样品堆积（FASS）的动态进样来提高该 MSPE-CZE 方法的灵敏度。在这种最佳条件下，8 种目标分析物的检测限范围为 0.015 至 0.105μg/mL。通过分析尿液样本，证明了 MSPE-CZE 在非法药物监测中的应用可行性，加标样品中目标药物的回收率范围为 85.4%至 110.1%。通过评估日内和日间精密度，测试了方法的重现性，得到的相对标准偏差分别小于 10.3%和 12.4%。为了提高分析的通量，使用了一种自制的 MSPE 阵列，该阵列具有同时处理 96 个样本的潜力。
