Escote-Carlson L J, Gabay-Laughnan S, Laughnan J R
Biotechnology Laboratory, University of British Columbia, Vancouver, Canada.
Mol Gen Genet. 1990 Sep;223(3):457-64. doi: 10.1007/BF00264454.
A WF9 strain of maize with the RD subtype of the S male-sterile cytoplasm (CMS-S) was converted to the inbred M825 nuclear background by recurrent backcrossing. The organization of the mitochondrial genomes of the F1 and succeeding backcross progenies was analyzed and compared with the progenitor RD-WF9 using probes derived from the S1 and S2 mitochondrial episomes, and probes containing the genes for cytochrome c oxidase subunit I (coxI), cytochrome c oxidase subunit II (coxII) and apocytochrome b (cob). Changes in mitochondrial DNA (mtDNA) organization were observed for S1-, S2-, and coxI-homologous sequences that involve loss of homologous restriction enzyme fragments present in the RD-WF9 progenitor. With the coxI probe, the loss of certain fragments was accompanied by the appearance of a fragment not detectable in the progenitor. The changes observed indicate the effect of the nuclear genome on the differential replication of specific mitochondrial subgenomic entities.
通过反复回交，将具有S型雄性不育细胞质（CMS-S）的RD亚型的玉米WF9品系转育到自交系M825的核背景中。使用源自S1和S2线粒体附加体的探针，以及包含细胞色素c氧化酶亚基I（coxI）、细胞色素c氧化酶亚基II（coxII）和脱辅基细胞色素b（cob）基因的探针，分析并比较了F1及其后续回交后代线粒体基因组的组织形式，并与亲本RD-WF9进行了比较。观察到S1、S2和coxI同源序列的线粒体DNA（mtDNA）组织形式发生了变化，这些变化涉及RD-WF9亲本中存在的同源限制性酶切片段的缺失。使用coxI探针时，某些片段的缺失伴随着一个在亲本中无法检测到的片段的出现。观察到的这些变化表明了核基因组对特定线粒体亚基因组实体差异复制的影响。
