Moredun Research Institute, Midlothian, UK.
Parasite Immunol. 2012 May;34(5):285-95. doi: 10.1111/j.1365-3024.2011.01317.x.
Infections with parasitic nematodes are of significant welfare and economic importance worldwide, and because of the emergence of anthelmintic resistance, this has lead to alternative methods of parasite control being required. Vaccination offers a feasible alternative control, and the majority of research has focused on the production of recombinant versions of native antigens previously identified as protective in vaccinated animals. Attempts at the production of protective recombinant subunit vaccines have been hindered, however, as these antigens have invariably failed to replicate the same level of protective immune response as seen with the native versions. It has been proposed that these failures are owing to the fact that the recombinant proteins do not contain the appropriate post-translational modifications to retain the protective capacity of the native molecules. In this review, we discuss a novel approach to vaccine antigen identification through the application of random peptide phage-display libraries and their use to identify peptide sequences that potentially mimic the structure(s) of antigenic epitopes. This area of research is still relatively novel with respect to parasites, and the current state of the art will be discussed here.
寄生虫线虫感染具有重要的福利和经济意义，并且由于驱虫剂耐药性的出现，这导致需要替代的寄生虫控制方法。疫苗接种提供了一种可行的替代控制方法，大多数研究都集中在生产先前被鉴定为接种动物保护性的天然抗原的重组版本上。然而，生产保护性重组亚单位疫苗的尝试受到了阻碍，因为这些抗原始终未能复制与天然版本相同水平的保护性免疫反应。有人提出，这些失败是由于重组蛋白不含适当的翻译后修饰来保持天然分子的保护能力。在这篇综述中，我们通过应用随机肽噬菌体展示文库讨论了一种疫苗抗原鉴定的新方法，并利用该方法鉴定了可能模拟抗原表位结构的肽序列。就寄生虫而言，这一研究领域相对较新，本文将讨论该领域的最新技术。
