血竭种子的自由基清除能力和还原能力。

Free radical scavenging and reducing power of Lawsonia inermis L. seeds.

机构信息

Pharmaceutical Chemistry Division, School of Advanced Sciences, VIT University, Vellore 632014, India.

出版信息

Asian Pac J Trop Med. 2011 Jun;4(6):457-61. doi: 10.1016/S1995-7645(11)60125-9.

DOI:10.1016/S1995-7645(11)60125-9

PMID:21771698

Abstract

OBJECTIVE

To determine the antioxidant activity, total phenolic and flavonoid content of Petroleum ether extract (PE), Dichloromethane extract (DCM), Ethanol extract (ET) and aqueous extract (AQ) of henna seeds.

METHODS

Total antioxidant assay (phosphomolybenum method), DPPH radical scavenging assay, reducing power assay and lipid peroxidation inhibition assay were used to ascertain the potential of seeds as an antioxidant.

RESULTS

In all the assays carried out ET showed a greater potential to scavenge DPPH radical, reduce MO (VI) to MO (V) complex and Fe (III) to Fe (II) and to inhibit lipid peroxidation. The IC(50) of ET was far greater than that of the standard, ascorbic acid (AS) in the lipid peroxidation assay. The activity of AQ was lesser when compared with that of ET but greater than PE and DCM. The amount of phenolics and flavonoids were present in higher amounts in ET followed by AQ. Trace amounts of phenolics were detected in PE and DCM, but the amount of flavonoids were below the detection level. The study showed that the antioxidant activity and the concentrations of phenolics and flavonoids are proportionate to each other.

CONCLUSIONS

Ethanolic extract of henna seeds are efficient antioxidants, which can be utilized for further isolation of active compounds and pharmaceutical applications.

摘要

目的

测定指甲花种子的石油醚提取物（PE）、二氯甲烷提取物（DCM）、乙醇提取物（ET）和水提物（AQ）的抗氧化活性、总酚和类黄酮含量。

方法

采用总抗氧化测定法（磷钼酸盐法）、DPPH 自由基清除测定法、还原力测定法和脂质过氧化抑制测定法来确定种子作为抗氧化剂的潜力。

结果

在所有进行的测定中，ET 显示出更大的潜力来清除 DPPH 自由基、将 MO（VI）还原为 MO（V）络合物和 Fe（III）还原为 Fe（II）以及抑制脂质过氧化。ET 的 IC（50）远大于脂质过氧化测定中的标准抗坏血酸（AS）。AQ 的活性与 ET 相比较小，但大于 PE 和 DCM。ET 中酚类和类黄酮的含量较高，其次是 AQ。PE 和 DCM 中检测到痕量的酚类，但类黄酮的含量低于检测水平。研究表明，抗氧化活性和酚类及类黄酮的浓度是相互成正比的。