Institute of Fermentation Engineering, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, PR China.
J Antibiot (Tokyo). 2011 Aug;64(8):563-9. doi: 10.1038/ja.2011.55. Epub 2011 Jul 20.
The potential of tautomycin to control oilseed rape stem rot was investigated in this paper. Tautomycin produced by Streptomyces spiroverticillatus strongly inhibited Sclerotinia sclerotiorum, which causes oilseed rape stem rot. Tautomycin showed great inhibition of the mycelial growth of S. sclerotiorum on potato dextrose agar (PDA) plates. The values of EC(50) and MIC were 3.26 × 10(-4) mM and 6.52 × 10(-4) mM, respectively. Tautomycin treatment also resulted in morphological abnormalities of S. sclerotiorum such as hyphal swellings and abnormally branched shapes, which were observed microscopically. Sclerotia of S. sclerotiorum soaked in the tautomycin solution for 24 h remained viable, but their ability to undergo myceliogenic germination on PDA plates was completely inhibited when the concentration of tautomycin reached 6.52 × 10(-4) mM. Tautomycin-treated oilseed rape leaves were found to have a low incidence of leaf blight caused by S. sclerotiorum. The activity of the protein phosphatase (PP) in S. sclerotiorum decreased by 41.6% and 52.6% when treated with 3.30 × 10(-4) mM and 6.52 × 10(-4) mM tautomycin, respectively. Cellular constituents also leaked from S. sclerotiorum cells incubated with tautomycin. The results suggest that the antimicrobial activity of tautomycin is due to the inhibition of the PP and then a change of membrane permeability. This paper also investigated related compounds that possess either a maleic anhydride or maleic acid moiety. Results showed 2,3-dimethylmaleic anhydride, diphenylmaleic anhydride and dimethyl maleate demonstrated significant activity against S. sclerotiorum. The values of EC(50) for these three compounds were 0.31 mM, 0.15 mM and 3.99 mM, respectively. The MIC values obtained for these compounds were 1.11 mM, 0.56 mM and 9.58 mM, respectively.
本文研究了 tautomycin 控制油菜茎腐病的潜力。由链霉菌 spiroverticillatus 产生的 tautomycin 强烈抑制了引起油菜茎腐病的核盘菌。tautomycin 在马铃薯葡萄糖琼脂（PDA）平板上对 S. sclerotiorum 的菌丝生长表现出很强的抑制作用。EC(50)和 MIC 值分别为 3.26×10(-4)mM 和 6.52×10(-4)mM。tautomycin 处理还导致 S. sclerotiorum 的形态异常，如菌丝肿胀和异常分枝，这些异常在显微镜下观察到。浸泡在 tautomycin 溶液中的 S. sclerotiorum 菌核在 24 小时内仍保持活力，但当 tautomycin 浓度达到 6.52×10(-4)mM 时，其在 PDA 平板上进行菌丝生成萌发的能力完全被抑制。用 tautomycin 处理的油菜叶片发现由 S. sclerotiorum 引起的叶枯病发病率较低。当用 3.30×10(-4)mM 和 6.52×10(-4)mM tautomycin 处理时，核盘菌中的蛋白磷酸酶（PP）活性分别降低了 41.6%和 52.6%。细胞成分也从用 tautomycin 孵育的 S. sclerotiorum 细胞中泄漏出来。结果表明，tautomycin 的抗菌活性是由于 PP 的抑制，然后是膜通透性的改变。本文还研究了具有马来酸酐或马来酸部分的相关化合物。结果表明，2,3-二甲基马来酸酐、二苯马来酸酐和马来酸二甲酯对 S. sclerotiorum 表现出显著的活性。这三种化合物的 EC(50)值分别为 0.31mM、0.15mM 和 3.99mM。这些化合物的 MIC 值分别为 1.11mM、0.56mM 和 9.58mM。
