Xu Congying, Hou Yiping, Wang Jianxin, Yang Guangfu, Liang Xiaoyu, Zhou Mingguo
College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Pesticide, Jiangsu Province, Nanjing 210095, China.
College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Pesticide, Jiangsu Province, Nanjing 210095, China.
Pestic Biochem Physiol. 2014 Oct;115:32-8. doi: 10.1016/j.pestbp.2014.08.001. Epub 2014 Aug 20.
Benzothiostrobin is a novel strobilurin fungicide. In this study, baseline sensitivity of Sclerotinia sclerotiorum (Lib.) de Bary to benzothiostrobin was determined using 100 strains collected during 2012 and 2013 from different geographical regions in Jiangsu Province of China, and the average EC50 value was 0.0218 (± 0.0111)μg/mL for mycelial growth. After benzothiostrobin treatment, hyphae were contorted with offshoot of top increasing and cell membrane permeability increased markedly, while sclerotial production and oxalic acid content significantly decreased. Benzothiostrobin strongly inhibited mycelial respiration within 12h and the oxygen consumption of the mycelia could not be inhibited after 24h. On detached rapeseed leaves, the protective and curative activity test of benzothiostrobin suggested that benzothiostrobin had good control efficiency against S. sclerotiorum, and protective activity was better than curative activity. These results will contribute to us evaluating the potential of the new strobilurin fungicide benzothiostrobin for management of diseases caused by S. sclerotiorum and understanding the mode of action of benzothiostrobin against S. sclerotiorum.
苯并噻菌酯是一种新型甲氧基丙烯酸酯类杀菌剂。本研究利用2012年和2013年从中国江苏省不同地理区域采集的100株核盘菌菌株，测定了核盘菌对苯并噻菌酯的基线敏感性，菌丝生长的平均EC50值为0.0218（±0.0111）μg/mL。经苯并噻菌酯处理后，菌丝扭曲，顶部分支增多，细胞膜通透性显著增加，而菌核产量和草酸含量显著降低。苯并噻菌酯在12小时内强烈抑制菌丝呼吸，24小时后菌丝的耗氧量无法被抑制。在离体油菜叶片上，苯并噻菌酯的保护和治疗活性试验表明，苯并噻菌酯对核盘菌有良好的防治效果，且保护活性优于治疗活性。这些结果将有助于我们评估新型甲氧基丙烯酸酯类杀菌剂苯并噻菌酯对核盘菌引起的病害的防治潜力，并了解苯并噻菌酯对核盘菌的作用方式。
