Wang Yong, Sun Yang, Zhang Ying, Zhang Yinxing, Han Lirong, Zhang Xing, Feng Juntao
Research and Development Center of Biorational Pesticides, Northwest A & F University, Yangling 712100, Shaanxi, China.
Research and Development Center of Biorational Pesticides, Northwest A & F University, Yangling 712100, Shaanxi, China.
Pestic Biochem Physiol. 2017 Jan;135:82-88. doi: 10.1016/j.pestbp.2016.05.006. Epub 2016 May 31.
Propamidine is an aromatic diamidine compound. In the current study, baseline sensitivity of Sclerotinia sclerotiorum to propamidine was determined using 78 strains collected from the oilseed rape fields without a previous history of propamidine usage. The median effective concentration (EC) values for propamidine inhibiting mycelial growth ranged from 0.406 to 3.647μg/mL, with a mean of 1.616±0.217μg/mL. There was no correlation between sensitivity to propamidine and sensitivity to dimethachlon or carbendazim. After treated with propamidine, mycelia were thinner with irregular distortion and more branches; cell wall became thicker with uneven distribution of cytoplasm than untreated control. In addition, sclerotia production, cell membrane permeability and oxalic acid content significantly decreased. On detached oilseed rape leaves, propamidine exhibited better control efficacy than carbendazim at the same concentration whether the leaves were inoculated with carbendazim-sensitive or resistant strains. All the results showed that propamidine exhibited strong antifungal activity and potential application in controlling S. sclerotiorum. Importantly, these data will provide more information on understanding the mode of action of propamidine against S. sclerotiorum and should be valuable for development of new antifungal drugs.
丙烷脒是一种芳香族二脒化合物。在本研究中，使用从没有丙烷脒使用历史的油菜田中收集的78个菌株，测定了核盘菌对丙烷脒的基线敏感性。丙烷脒抑制菌丝体生长的半数有效浓度（EC）值范围为0.406至3.647μg/mL，平均值为1.616±0.217μg/mL。对丙烷脒的敏感性与对菌核净或多菌灵的敏感性之间没有相关性。用丙烷脒处理后，菌丝体更细，有不规则扭曲和更多分支；与未处理的对照相比，细胞壁变厚，细胞质分布不均匀。此外，菌核产生、细胞膜通透性和草酸含量显著降低。在离体油菜叶片上，无论叶片接种的是对多菌灵敏感还是抗性菌株，在相同浓度下，丙烷脒的防治效果均优于多菌灵。所有结果表明，丙烷脒对核盘菌表现出较强的抗真菌活性和潜在的防治应用价值。重要的是，这些数据将为理解丙烷脒对核盘菌的作用方式提供更多信息，对新型抗真菌药物的开发具有重要价值。
