Haghbeen K, Pourmolaei S, Mareftjo M J, Mousavi A, Akbari Noghabi K, Hosseini Shirazi F, Meshkat A
Biochemistry Department, The National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965-161, Tehran, Iran.
J Biomed Biotechnol. 2011;2011:165852. doi: 10.1155/2011/165852. Epub 2011 Jun 27.
In pursuit of strong shikalkin-producing cell lines, seeds of the Iranian Arnebia euchroma were collected from Dena altitudes in the central Zagross. Chemical analysis showed that the dried root of the plant contained about 8.5% (w/w) shikalkin pigment. The root explants of the young plantlets, obtained from the germinated seeds, were used for establishing callus. Then, parameters effective on proliferation and pigment production of the resulting calli were studied in detail. Accordingly, two modified media called mLS and mM9 were optimized for propagation and pigment production, respectively. Using these media, the biomass of the A. euchroma calli was increased to 600%, and the pigment production reached to a maximum of 16.3 mg per gram of the wet biomass in a period of a subculture (21 days). Parallel to these experiments, the antimicrobial activity of shikalkin pigment was examined on some fungi and gram-positive and gram-negative bacteria. Results indicated that the pigment was almost ineffective on fungi and gram-negative bacteria, but it was meaningfully effective against Micrococcus luteus.
为了获得高产紫草素的细胞系，从扎格罗斯中部海拔地区的代纳采集了伊朗紫草根的种子。化学分析表明，该植物的干燥根中含有约8.5%（w/w）的紫草素色素。从发芽种子获得的幼苗根外植体用于建立愈伤组织。然后，详细研究了对所得愈伤组织增殖和色素产生有效的参数。因此，分别针对增殖和色素产生优化了两种改良培养基，称为mLS和mM9。使用这些培养基，紫草根愈伤组织的生物量增加到600%，在一次继代培养（21天）期间，色素产量最高达到每克湿生物量16.3毫克。在进行这些实验的同时，还检测了紫草素色素对一些真菌以及革兰氏阳性和革兰氏阴性细菌的抗菌活性。结果表明，该色素对真菌和革兰氏阴性细菌几乎无效，但对藤黄微球菌有显著效果。
