Equine Diseases Study Group (COPAR, GI-2120), Animal Pathology Department, Veterinary Faculty, Santiago de Compostela University, 27002 Lugo, Spain.
Biomed Res Int. 2013;2013:567876. doi: 10.1155/2013/567876. Epub 2013 Apr 21.
Helminth zoonoses are parasitic infections shared by humans and animals, being the soil-transmitted helminths (STHs) mainly caused by roundworms (ascarids) and hookworms. This study was aimed to assess the individual and/or mixed production of two helminth-antagonistic fungi, one ovicide (Mucor circinelloides) and other predator (Duddingtonia flagrans). Fungi were grown both in Petri plates and in a submerged culture (composed by water, NaCl, Na2HPO4 · 12 H2O, and wheat (Triticum aestivum)). A Fasciola hepatica recombinant protein (FhrAPS) was incorporated to the cultures to improve fungal production. All the cultured plates showed fungal growth, without difference in the development of the fungi when grown alone or mixed. High counts of Mucor spores were produced in liquid media cultures, and no significant differences were achieved regarding single or mixed cultures, or the incorporation of the FhrAPS. A significantly higher production of Duddingtonia spores after the incorporation of the FhrAPS was observed. When analyzing the parasiticide efficacy of the fungal mixture, viability of T. canis eggs reduced to 51%, and the numbers of third stage cyathostomin larvae reduced to 4%. It is concluded, the capability of a fungal mixture containing an ovicide (Mucor) and a predator species (Duddingtonia) for growing together in a submerged medium containing the FhrAPS offers a very interesting tool for preventing STHs.
人体寄生虫病是人类和动物共患的寄生虫感染，其中土壤传播的蠕虫（STHs）主要由蛔虫（蛔虫）和钩虫引起。本研究旨在评估两种具有驱虫作用的真菌（一种杀卵真菌[环毛霉]和另一种捕食真菌[杜氏藻]）的单独和/或混合生产。真菌在培养皿和浸没培养物中生长（由水、NaCl、Na2HPO4·12 H2O 和小麦[小麦]组成）。将一种 Fasciola hepatica 重组蛋白（FhrAPS）掺入培养物中以提高真菌产量。所有培养的平板均显示出真菌生长，无论是单独生长还是混合生长，真菌的发育都没有差异。在液体培养基中培养物中产生了大量的毛霉孢子，并且在单独或混合培养物或掺入 FhrAPS 方面没有差异。观察到掺入 FhrAPS 后杜氏藻孢子的产量显著增加。在分析真菌混合物的驱虫功效时，犬绦虫卵的存活率降低到 51%，第三期旋毛虫幼虫的数量减少到 4%。结论是，含有杀卵真菌（毛霉）和捕食真菌（杜氏藻）的真菌混合物在含有 FhrAPS 的浸没培养基中一起生长的能力为预防 STHs 提供了一种非常有趣的工具。
