SONAS EA 921, IFR 149, Quasav UFR des Sciences Pharmaceutiques et d'Ingénierie de la Santé, 16 Bd Daviers, 49100 Angers, France.
Phytochem Anal. 2012 Mar-Apr;23(2):152-8. doi: 10.1002/pca.1336. Epub 2011 Jul 19.
The Derris genus is known to contain flavonoid derivatives, including prenylated flavanones and isoflavonoids such as rotenoids, which are generally associated with significant biological activity.
To develop an efficient preparative isolation procedure for bioactive cajaflavanone.
Fast centrifugal partition chromatography (FCPC) was optimised to purify cajaflavanone from Derris ferruginea stems in a single step as compared to fractionation from the cyclohexane extract by successive conventional solid-liquid chromatography procedures. The purification yield, purity, time and solvent consumption per procedure are described. The anti-fungal, anti-bacterial, anti-leishmanial, anti-plasmodial, anti-oxidant activities and the inhibition of advanced glycation end-products (AGEs) by cajaflavanone accumulation are described.
FCPC enabled cajaflavanone purification in a single separation step, yielding sufficient quantities to perform in vitro biological screening. Interestingly, cajaflavanone had an inhibitory effect on the formation of AGEs, without displaying any in vitro anti-oxidant activity.
A simple and efficient procedure, in comparison with other preparative methods, for bioactive cajaflavone purification has been developed using FCPC.
鱼藤属植物被认为含有类黄酮衍生物，包括prenylated flavanones 和异黄酮类化合物如鱼藤酮，这些化合物通常与显著的生物活性有关。
开发一种从鱼藤茎中高效制备生物活性鱼藤酮的分离方法。
与传统的固液色谱分离方法相比，优化快速离心分配色谱（FCPC）以一步法从鱼藤根中分离纯化鱼藤酮。描述了每个步骤的纯化产率、纯度、时间和溶剂消耗。描述了鱼藤酮的抗真菌、抗细菌、抗利什曼原虫、抗疟原虫、抗氧化活性以及对晚期糖基化终产物（AGEs）的抑制作用。
FCPC 能够在单个分离步骤中纯化鱼藤酮，得到足够的量进行体外生物筛选。有趣的是，鱼藤酮对 AGEs 的形成具有抑制作用，而没有显示任何体外抗氧化活性。
与其他制备方法相比，使用 FCPC 开发了一种简单有效的生物活性鱼藤酮纯化方法。
