Biomaterials Research Center, South China University of Technology, Guangzhou 510640, PR China.
ACS Appl Mater Interfaces. 2011 Aug;3(8):2885-94. doi: 10.1021/am2004398. Epub 2011 Jul 20.
In this article, we present the first report on the antibacterial activity and cytotoxicity of poly(amidoamine) (PAMAM) dendrimers immobilized on three types of titanium-based substrates with and without calcium phosphate coating. We show that the amino-terminated PAMAM dendrimers modified with various percentages (0-60%) of poly(ethylene glycol) (PEG) strongly adsorbed on the titanium-based substrates. The resultant dendrimer films effectively inhibited the colonization of the Gram-negative bacteria Pseudomonas aeruginosa (strain PAO1) and, to a lesser extent, the Gram-positive bacteria Staphylococcus aureus (SA). The antibacterial activity of the films was maintained even after storage of the samples in PBS for up to 30 days. In addition, the dendrimer films had a low cytotoxicity to human bone mesenchymal stem cells (hMSCs) and did not alter the osteoblast gene expression promoted by the calcium phosphate coating.
本文首次报道了固定在三种不同钛基基底上的聚酰胺-胺（PAMAM）树枝状聚合物的抗菌活性和细胞毒性，基底有无磷酸钙涂层。结果表明，经不同百分比（0-60%）聚乙二醇（PEG）修饰的氨基末端 PAMAM 树枝状聚合物强烈吸附在钛基基底上。所得树枝状聚合物薄膜能有效抑制革兰氏阴性菌铜绿假单胞菌（PAO1 株）和革兰氏阳性菌金黄色葡萄球菌（SA）的定植。即使将样品在 PBS 中储存长达 30 天，薄膜的抗菌活性也能保持。此外，树枝状聚合物薄膜对人骨髓间充质干细胞（hMSCs）的细胞毒性较低，且不改变磷酸钙涂层促进成骨细胞基因表达的作用。
