Molina Noemi, González Ana, Monopoli Donato, Mentado Belinda, Becerra José, Santos-Ruiz Leonor, Vida Yolanda, Perez-Inestrosa Ezequiel
Universidad de Málaga - IBIMA, Dpto. Química Orgánica, Campus de Teatinos s/n, 29071 Málaga, Spain.
Centro Andaluz de Nanomedicina y Biotecnología-BIONAND. Parque Tecnológico de Andalucía, c/ Severo Ochoa, 35, 29590 Campanillas, Málaga, Spain.
Polymers (Basel). 2020 Apr 1;12(4):770. doi: 10.3390/polym12040770.
Osseointegration of metal prosthetic implants is a yet unresolved clinical need that depends on the interplay between the implant surface and bone cells. The lack of a relationship between bone cells and metal has traditionally been solved by coating the former with "organic" ceramics, such as hydroxyapatite. A novel approach is hereby presented, immobilizing covalently dendrimeric structures onto titanium implants. Amide-based amino terminal dendrons were synthetized and coupled to titanium surfaces in a versatile and controlled way. The dendritic moieties provide an excellent scaffold for the covalent immobilization of bioactive molecules, such as extracellular matrix (ECM) protein components or antibiotics. Herein, tripeptide arginine-glycine-aspartic acid (RGD) motifs were used to decorate the dendritic scaffolds and their influence on cell adhesion and proliferation processes was evaluated.
金属假体植入物的骨整合是一个尚未解决的临床需求，它取决于植入物表面与骨细胞之间的相互作用。传统上，骨细胞与金属之间缺乏相互作用的问题是通过用“有机”陶瓷（如羟基磷灰石）涂覆骨细胞来解决的。本文提出了一种新方法，即将树枝状结构共价固定在钛植入物上。合成了基于酰胺的氨基末端树枝状分子，并以通用且可控的方式将其与钛表面偶联。树枝状部分为生物活性分子（如细胞外基质（ECM）蛋白成分或抗生素）的共价固定提供了一个极好的支架。在此，使用三肽精氨酸 - 甘氨酸 - 天冬氨酸（RGD）基序来修饰树枝状支架，并评估了它们对细胞黏附和增殖过程的影响。
