Department of Biomedicine and Biotechnology, University of Alcalá, 28805 Madrid, Spain.
Department of Organic and Inorganic Chemistry, and Research Institute in Chemistry "Andrés M. del Río" (IQAR), University of Alcalá, 28805 Madrid, Spain.
Biomolecules. 2019 Aug 23;9(9):405. doi: 10.3390/biom9090405.
Antibiotic resistance is currently one of the main threats to public health security. Biofilm formation is a resistance mechanism that is responsible for most human bacterial infections and requires new and effective therapeutic approaches, such as those provided by nanotechnology. In this work, the antibacterial effect of carbosilane metallodendrimers with different metals (copper(II) and ruthenium(II)), ligands (chloride and nitrate) and generations (generation 0, 1 and 2) has been studied using planktonic Gram-positive () and Gram-negative () bacteria. Furthermore, the ability of the metallodendrimers to avoid the formation of biofilms was also evaluated. The results showed a promising biocide activity in both types of planktonic bacteria, especially for first-generation dendrimers, which arises from the metal complexation to the dendrimer. Cu(II) metallodendrimers require lower concentration than Ru(II) counterpart to inhibit the production of biofilms, but none produce hemolysis at the inhibitory concentrations and can be safely used as antibacterial agents. In particular, the first-generation Cu(II) metallodendrimer with nitrate ligands displayed the most promising properties to continue with further studies in both planktonic cells and biofilms.
抗生素耐药性是目前对公共卫生安全的主要威胁之一。生物膜形成是导致大多数人类细菌感染的一种耐药机制，需要新的有效治疗方法，例如纳米技术提供的方法。在这项工作中，使用浮游革兰氏阳性（）和革兰氏阴性（）细菌研究了具有不同金属（铜（II）和钌（II））、配体（氯和硝酸盐）和代（0、1 和 2）的碳硅烷金属树状大分子的抗菌作用。此外，还评估了金属树状大分子避免生物膜形成的能力。结果表明，两种浮游细菌都表现出有希望的杀菌活性，特别是第一代树突状大分子，这源于金属与树突状大分子的络合。Cu(II)金属树突状大分子需要比 Ru(II)对应物更低的浓度来抑制生物膜的产生，但在抑制浓度下均不会产生溶血，并且可以安全用作抗菌剂。特别是具有硝酸盐配体的第一代 Cu(II)金属树突状大分子在浮游细胞和生物膜中进一步研究中表现出最有前途的特性。
