Okazaki M, Ohmae H, Takahashi J, Kimura H, Sakuda M
Department of Dental Technology, Osaka University Faculty of Dentistry, Japan.
Biomaterials. 1990 Oct;11(8):568-72. doi: 10.1016/0142-9612(90)90080-a.
To examine the response to biological hard tissues, a carbonate-containing hydroxyapatite with chemical composition and crystallinity similar to those of bone was synthesized at pH 7.4 and 60 degrees C. The apatite powder was mixed with collagen solution, whose antigenicity had been removed by enzymatic treatment, and formed into apatite-collagen pellets. After insolubilization by UV-irradiation for 4 h, the composites showed remarkably reduced disintegration and maintained their shape under 3.6 MPa of stress after 1 wk incubation in 0.9% NaCl solution. They showed good biocompatibility when implanted beneath the periosteum cranii of rats. The UV-irradiated sample kept its features well and was packed with newly created material 3 wk after implantation.
为了研究对生物硬组织的反应，在pH 7.4和60℃条件下合成了一种化学成分和结晶度与骨相似的含碳酸盐羟基磷灰石。将磷灰石粉末与经酶处理已去除抗原性的胶原溶液混合，制成磷灰石 - 胶原微丸。经紫外线照射4小时使其固化后，复合材料的崩解明显减少，在0.9%氯化钠溶液中孵育1周后，在3.6 MPa应力下仍能保持其形状。将其植入大鼠颅骨骨膜下时显示出良好的生物相容性。紫外线照射的样品在植入3周后仍能很好地保持其特征，并充满了新生成的物质。
