Center for Biofluid and Biomimic Research, Pohang University of Science and Technology, Pohang, Republic of Korea.
Biomaterials. 2011 Oct;32(29):7191-9. doi: 10.1016/j.biomaterials.2011.05.023. Epub 2011 Jul 20.
Time-resolved dynamic imaging of bio-fluids can provide valuable information for clinical diagnosis and treatment of circulatory disorders. Quantitative information on non-transparent blood flows can be directly obtained by particle-tracing dynamic X-ray imaging, which needs better spatial resolution and enhanced image contrast compared to static imaging. For that use, tracer particles tagging along the flow streams are critically required. In this study, taking the advantage of high X-ray absorption, gold nanoparticles (AuNPs) are incorporated into human red blood cells (RBC) to produce contrast-enhanced tracers designed for dynamic X-ray imaging of blood flows. RBCs are advantageous tracers for blood flow measurements since they are natural and primary components of blood. The loading efficiency of AuNPs into RBCs is investigated in terms of the surface properties of the AuNPs. The AuNP-incorporated RBC provides a potential in the dynamic X-ray imaging of blood flows which can be used for clinical applications.
生物流体的时间分辨动态成像是临床诊断和治疗循环障碍的重要手段。通过示踪动力学 X 射线成像，可以直接获得非透明血流的定量信息，与静态成像相比，这种方法需要更高的空间分辨率和增强的图像对比度。为此，需要沿流(stream)追踪示踪粒子(tracer particle)。在这项研究中，利用金纳米粒子（AuNPs）的高 X 射线吸收率，将其掺入人红细胞（RBC）中，以产生用于血流动态 X 射线成像的对比增强示踪剂。由于 RBC 是血液的天然和主要成分，因此它们是血流测量的优势示踪剂。从 AuNPs 的表面特性方面研究了 AuNPs 掺入 RBC 的负载效率。AuNP 掺入的 RBC 为血流的动态 X 射线成像提供了一种可能，可用于临床应用。
