Department of Gastroenterology, Southwest Hospital, The Third Military Medical University, Chongqing, China.
J Clin Pathol. 2011 Dec;64(12):1051-7. doi: 10.1136/jclinpath-2011-200211. Epub 2011 Jul 21.
The human protection of telomeres 1 (hPOT1) protein, a single-strand telomeric DNA binding protein, plays an important role in telomere protection and telomere length regulation. However, its effect on invasion of gastric cancer remains unclear.
To explore the role of hPOT1 in the proliferation and invasion of gastric cancer cells.
The gastric expression of hPOT1 was examined in normal gastric mucosa (n=25), intestinal metaplasia (n=20), gastric dysplasia (n=20) and gastric cancer (n=150) by immunohistochemistry. The mean optical density (MOD) of the immunostaining was determined by semi-quantitative image analysis. The role of hPOT1 in the cell proliferation, apoptosis and invasion of gastric cancer 7901 cells was determined by means of the RNA interference (RNAi) of hPOT1 mRNA. The effects of hPOT1 RNAi on the expression of hPinX1 and hTERT were detected with western blotting.
The hPOT1 MOD was progressively increased from the normal mucosa to intestinal metaplasia, dysplasia, and gastric cancer. An increased hPOT1 expression significantly correlated with tumour serosal invasion, node metastasis and advanced stage. Transfection of hPOT1 siRNA into SGC-7901 cells led to a decrease in cell proliferation, colony formation and invasion, and also an increase of apoptosis. An up-regulation of hPinX1 and down-regulation of hTERT were found in gastric cancer cells with hPOT1 siRNA.
Increased hPOT1 expression is associated with an advanced tumour stage. hPOT1 RNAi inhibits proliferation and invasion, and induces apoptosis of gastric cancer cells. The effects of hPOT1 RNAi seem to be functionally linked to up-regulation of PinX1 and down-regulation of hTERT.
端粒 1 （hPOT1）蛋白是一种单链端粒 DNA 结合蛋白，在保护端粒和调节端粒长度方面发挥着重要作用。然而，其对胃癌侵袭的影响尚不清楚。
探讨 hPOT1 在胃癌细胞增殖和侵袭中的作用。
采用免疫组织化学法检测 25 例正常胃黏膜、20 例肠上皮化生、20 例胃上皮不典型增生和 150 例胃癌组织中 hPOT1 的表达，用半定量图像分析方法测定免疫组化染色的平均光密度（MOD）。采用 RNA 干扰（RNAi）技术沉默 hPOT1mRNA，观察 hPOT1 对胃癌 7901 细胞增殖、凋亡和侵袭的影响，并用 Western blot 检测 hPOT1RNAi 对 hPinX1 和 hTERT 表达的影响。
hPOT1MOD 从正常黏膜到肠上皮化生、不典型增生、胃癌逐渐增加。hPOT1 表达增加与肿瘤浆膜侵犯、淋巴结转移和晚期密切相关。转染 hPOT1siRNA 至 SGC-7901 细胞后，细胞增殖、集落形成和侵袭能力降低，凋亡增加。胃癌细胞中 hPOT1siRNA 可上调 hPinX1，下调 hTERT。
hPOT1 表达增加与肿瘤进展有关。hPOT1RNAi 抑制胃癌细胞增殖和侵袭，诱导凋亡。hPOT1RNAi 的作用似乎与 PinX1 的上调和 hTERT 的下调功能相关。
