Mei Peng-Jin, Chen Yan-Su, Du Ying, Bai Jin, Zheng Jun-Nian
Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou, 221002, Jiangsu Province, China.
Med Oncol. 2015 Mar;32(3):73. doi: 10.1007/s12032-015-0545-7. Epub 2015 Feb 20.
PinX1 induces apoptosis and suppresses cell proliferation in some cancer cells, and the expression of PinX1 is frequently decreased in some cancer and negatively associated with metastasis and prognosis. However, the precise roles of PinX1 in gliomas have not been studied. In this study, we found that PinX1 obviously reduced the gliomas cell proliferation through regulating the expressions of cell cycle-relative molecules to arrest cell at G1 phase and down-regulating the expression of component telomerase reverse transcriptase (hTERT in human), which is the hardcore of telomerase. Moreover, PinX1 could suppress the abilities of gliomas cell wound healing, migration and invasion via suppressing MMP-2 expression and increasing TIMP-2 expression. In conclusion, our results suggested that PinX1 may be a potential suppressive gene in the progression of gliomas.
PinX1可诱导某些癌细胞凋亡并抑制其增殖，在一些癌症中PinX1的表达常降低，且与转移和预后呈负相关。然而，PinX1在胶质瘤中的具体作用尚未见研究报道。本研究发现，PinX1通过调节细胞周期相关分子的表达使细胞停滞于G1期，从而明显降低胶质瘤细胞的增殖，同时下调端粒酶核心组分端粒酶逆转录酶（人类为hTERT）的表达。此外，PinX1可通过抑制MMP-2表达、增加TIMP-2表达来抑制胶质瘤细胞的伤口愈合、迁移及侵袭能力。总之，我们的结果提示PinX1可能是胶质瘤进展过程中的一个潜在抑癌基因。
