Lucio B, Fabricant J
Department of Avian and Aquatic Animal Medicine, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Avian Dis. 1990 Oct-Dec;34(4):865-70.
Three virus isolates (ECV-1, -2, and -3) recovered from cloacae of chickens in flocks that experienced drops in egg production were identified as infectious bronchitis virus (IBV), based on characteristic embryo lesions, chloroform sensitivity, coronavirus morphology, and serology. Because these isolates were recovered from the cloacae of the hens, their tissue tropism was compared with the prototype strain of IBV, Massachusetts-41 (M-41), in experimentally inoculated chickens. During the 39-day period postinoculation (PI), virus isolation was attempted from digestive and respiratory tracts, kidney, and cloacal swabs. ECV-1, ECV-2, and M-41 were more frequently recovered from the cecal tonsils than from other tissues. ECV-1, ECV-3, and M-41 were also recovered from kidney for up to 39 days PI. ECV-2 and ECV-3 had a limited distribution in respiratory tissues, being isolated only sporadically from trachea, bronchus, and lung. Surprisingly, ECV-2 was isolated from esophagus at 2, 16, 30, and 39 days PI; otherwise, its distribution in other tissues was sporadic. Results confirmed that IBV, including M-41, can infect a variety of tissues and that some isolates may be recovered frequently from digestive tract tissues, particularly from the cecal tonsils.
从产蛋量下降鸡群的泄殖腔中分离出的三种病毒毒株（ECV-1、-2和-3），根据其特征性的胚胎病变、对氯仿的敏感性、冠状病毒形态以及血清学鉴定为传染性支气管炎病毒（IBV）。由于这些毒株是从母鸡的泄殖腔中分离出来的，因此在实验接种的鸡中，将它们的组织嗜性与IBV的原型毒株马萨诸塞-41（M-41）进行了比较。在接种后39天内，尝试从消化道、呼吸道、肾脏和泄殖腔拭子中分离病毒。ECV-1、ECV-2和M-41从盲肠扁桃体中分离出来的频率高于其他组织。在接种后长达39天的时间里，ECV-1、ECV-3和M-41也能从肾脏中分离出来。ECV-2和ECV-3在呼吸道组织中的分布有限，仅偶尔从气管、支气管和肺中分离出来。令人惊讶的是，在接种后第2、16、30和39天从食管中分离出了ECV-2；否则，它在其他组织中的分布是零星的。结果证实，包括M-41在内的IBV可以感染多种组织，并且一些毒株可能经常从消化道组织中分离出来，特别是从盲肠扁桃体中。
